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Primary Antibodies  >  Phospho Specific Antibodies
 

AKT pT308 Monoclonal Antibody Biotin Conjugated

Mouse Monoclonal 18F3.H11 IgG1 kappa
Code

200-306-269
Size

50 µg
Price

$239.00
Availability

Ships next business day.
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Western Blot of Mouse anti-Akt phospho T308 Biotin Conjugated antibody.  
Lane 1:  GST tagged AKT1 active recombinant protein.  
Lane 2:  GST tagged AKT1 un-active recombinant protein.  
Load:  25 ng per lane.
Primary antibody:  Akt phospho T308 Biotin Conjugated antibody at 1:1,000 for overnight at 4°C.
Secondary antibody:  HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT.
Block:  MB-070 for 30 min at RT.
Predicted/Observed size:  79 kDa, 79 kDa for Akt phospho T308. 
Other band(s): none
Western Blot of Mouse anti-Akt phospho T308 Biotin Conjugated antibody.  
Lane 1:  GST tagged AKT1 active recombinant protein.  
Lane 2:  GST tagged AKT1 un-active recombinant protein.  
Load:  25 ng per lane.
Primary antibody:  Akt phospho T308 Biotin Conjugated antibody at 1:1,000 for overnight at 4°C.
Secondary antibody:  HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT.
Block:  MB-070 for 30 min at RT.
Predicted/Observed size:  79 kDa, 79 kDa for Akt phospho T308. 
Other band(s): none
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Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1, Akt phospho T308 Antibody, Anti-AKT pT308 Monoclonal Antibody Biotin Conjugated

AKT pT308 Monoclonal Antibody Biotin Conjugated Properties

Description
Anti-AKT pT308 (MOUSE) Monoclonal Antibody Biotin Conjugated - 200-306-269
Host
Mouse
Target Species
Human
Known Cross Reactivity
human, mouse, rat, monkey
Clonality
Monoclonal 18F3.H11 IgG1 kappa
Application
ELISA : 1:20,000
FlowCytometry : User Optimized
Western Blot : 1:500 - 1:3,000
Immunochemistry: 20 µg/ml
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG1
Label
FP Value
10-20 moles Biotin per mole of IgG1
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
GeneName
AKT1
Related Links
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AKT pT308 Monoclonal Antibody Biotin Conjugated Description

Background
Anti-AKT phospho T308 is ideal for western blotting, ELISA, IHC and IP. Phospho AKT pT308 antibody is specific for AKT protein phosphorylated at T308. AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. Anti-AKT pT308 monoclonal antibody is ideal for investigators involved in Cancer, Cell Signaling, Neuroscience, Signal Transduction research.
Immunogen
Anti-AKT pT308 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Biotin Conjugated Anti-AKT pT308 is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pT308 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.
Purity/Specifity
Anti-AKT pT308 was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at T308. A BLAST analysis was used to suggest cross-reactivity with AKT pT308 from most vertebrate species sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 will likely occur. Cancer, Cell Signaling, Neuroscience, Signal Transduction research.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Lawlor, M. A. and Alessi, D.R. (2001). PKB/AKT: a key mediator of cell proliferation, survival and insulin responses. J. Cell Science 114:2903-2910. Alessi, D. R. (2001). Discovery of PDK1, one of the missing links in insulin signal transduction. Biochem. Soc. Trans. 29,1 -14. Jones,P.F., Jakubowicz,T., Pitossi,F.J., Maurer,F. and Hemmings,B.A. (1991) Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily. Proc. Natl. Acad. Sci. U.S.A. 88 (10), 4171-4175.
Specific Reference
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Product Type
Proteins and Peptides;
Catalog Number
000-000-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, chicken
Catalog Number
100-401-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-269

Product Label

 

Description
Biotin
Color
n/a
Conjugation Reference
Modified from Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
Molecular Weight
455
Excitation Wavelength
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester