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Gli1 Antibody

Rabbit Polyclonal
NCI Collaboration
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Immunohistochemistry using Rockland's anti-Gli-1 antibody shows detection of mouse Gli-1 present in transfected 293T cells (red). HEK293T cells were transiently transfected with Gli-1 (murine). Rockland’s Anti-Gli-1 antiserum (rabbit) was added 1:400, followed by a fluorescent labeled anti-rabbit IgG secondary. Personal communication, Tom Curran, Children’s Hospital of Philadelphia, Philadelphia, PA.
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Number:
100-401-223S
Size:
25 µL
Price:
$95.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Gli1 Antibody Properties

Description
Anti-Gli1 (RABBIT) Antibody - 100-401-223S
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
mouse, human
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunohistochemistry: 1:500 - 1:2,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
Antiserum
Label
Concentration
75 mg/ml by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
GLI1
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Gli1 Antibody Description

Background
Anti Gli1 Antibody was produced against a peptide corresponding to the carboxy-terminal region of the mouse Gli-1 protein. This region of Gli1 is not conserved among other gli family members, namely Gli-2 and Gli-3. Gli was termed by Kinzler et al. (1987) as 'glioma-associated oncogene' amplified in malignant gliomas. Analysis of the cloned gene demonstrates that the gene contains 5 repeats of zinc-finger sequences, which places Gli in the family of Kruppel (Kr) zinc finger proteins. Northern analysis reveals that Gli is expressed in embryonal carcinoma cells but not in most adult tissue. Gli has been localized to 12q13-q14.3 by Southern blot analysis. In mice, the gene is located on chromosome 10. In mice, three zinc finger transcription factors, Gli-1, Gli-2 and Gli-3, have been implicated in the transduction of Sonic hedgehog (Shh) signal. In papillary epithelium, shh, gli1 and ptc all follow similar expression patterns. Gli-1 expression is central and probably sufficient for tumor development in humans.
Synonyms
Zinc finger protein GLI1 antibody, Glioma-associated oncogene antibody, Oncogene GLI antibody
Immunogen
The whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acids 805-820 of mouse Gli-1. The peptide was synthesized as a multiple antigen peptide (MAP).
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 120 kDa in size corresponding to Gli-1 protein by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, perform heat mediated antigen retrieval via the microwave method before commencing with staining.
Purity/Specifity
This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. Reactivity is observed against Mouse and Human Gli-1. Cross-reactivity with other species is likely but has not been determined. No reaction occurs with human or mouse Gli-2 or Gli-3.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Hall, J.M., Hooper, J.E., Finger, T.E. (1999) Expression of sonic hedgehog, patched, and gli1 in developing taste papillae of the mouse. J. Comp. Neurol. 406(2):143-155. Park, H. L. et. al. (2000) Mouse Gli1 mutants are viable but have defects in SHH signaling in combination with a Gli2 mutation. Development 127(8): 1593-1605. Kinzler, K.W. et. al. (1987) Identification of an amplified, highly expressed gene in a human glioma. Science 236:70-73.
Specific Reference
Wetmore, C., Eberhardt, D. and Curran, T. (2000) The normal patched allele is expressed in medulloblastomas from mice with heterozygous germline mutation of patched. Cancer Res. 60(8): 2239-2246. Qualtrough D et al. (2004) Hedgehog signalling in colorectal tumour cells: induction of apoptosis with cyclopamine treatment. Int J Cancer 110:831-7. Niemann C et al. (2003) Indian hedgehog and beta-catenin signaling: role in the sebaceous lineage of normal and neoplastic mammalian epidermis. Proc Natl Acad Sci U S A 100 Suppl 1:11873-80.
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human, mouse, rat
Catalog Number
100-401-149

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
600-401-928

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry