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Primary Antibodies  >  Epigenetic Research Antibodies
 

Histone H3 Antibody

Rabbit Polyclonal
Code

100-401-E81
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Immunohistochemistry of Histone H3 antibody  
Tissue: Lung
Fixation:  formalin fixed paraffin embedded  
Antigen retrieval:  user optimized
Primary antibody:  100-401-E81 Histone H3 antibody  1:100 
Secondary antibody:  Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT
Image provided courtesy of Andrew Elston, LifeSpan BioSciences, Inc.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Histone H3.1, H3F, H3FA, H3FB, H3FL, H3FL, H3FC, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ, Histone 3 Antibody

Histone H3 Antibody Properties

Description
Anti-Histone H3 (Rabbit) Antibody - 100-401-E81
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat, chicken, dog, monkey, Xenopus laevis, Arabidopsis thaliana, Caenorhabditis elegans, Fruit fly
Clonality
Polyclonal
Application
ELISA : 1:10000
Western Blot : 1:2000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
83 mg/ml by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
GeneName
HIST1H3A
Related Links
Download CofA
Lot#  

Histone H3 Antibody Description

Background
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone proteins are highly post-translationally modified with Histone H3 being the most extensively modified of the five histones. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 Antibody is ideal for investigators involved in Cell Signaling, Epigenetics, Nuclear Signaling research and Signal Transduction research.
Immunogen
Anti-Histone-3 was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to the c-terminus region of human histone-3.
Immunogen Type
Peptide
Storage Condition
Store H3 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Histone H3 antibody has been tested for use in ELISA and western blot. For western blots expect a band of approximately 15.4 kDa in size corresponding tothe Histone 3 protein. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-Histone H3 is directed against the human histone 3 protein. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest reactivity with human and multiple other eukaryotic. Cross-reactivity with histone-3 from other sources have not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
C L Peterson, M A Laniel. (2004) Histones and histone modifications. Current Biology, Vol. 14, No. 14. (27 July 2004), pp. R546-R551, doi:10.1016/j.cub.2004.07.007. S. B. Hake, C. D. Allis. (2006) Histone H3 variants and their potential role in indexing mammalian genomes: the "H3 barcode hypothesis". Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 17. (25 April 2006), pp. 6428-6435, doi:10.1073/pnas.0600803103.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human
Catalog Number
600-401-879

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Drosophila melanogaster
Catalog Number
600-401-914

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
mammalian, avian
Catalog Number
600-403-939

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry