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Primary Antibodies  >  Neuroscience Antibodies
 

Sodium-Iodide Symporter Antibody

Mouse Monoclonal 14F IgG1
Code

200-301-G49
Size

100 µg
Price

$309.00
Availability

Ships within 2 weeks.
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Shipping:
$20.00 to United States
Handling:
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Synonyms: NIS, SLC5A5, solute carrier family 5, Na (+)I(-) cotransporter, Sodium/iodide cotransporter,

Sodium-Iodide Symporter Antibody Properties

Description
Anti-Sodium-Iodide Symporter (MOUSE) Monoclonal Antibody - 200-301-G49
Host
Mouse
Target Species
Human
Known Cross Reactivity
Human, Mouse, Rat
Clonality
Monoclonal 14F IgG1
Application
Western Blot : 1:1000
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG1
Label
Concentration
1mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer
50% (v/v) Glycerol
GeneName
SLC5A5
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Lot#  

Sodium-Iodide Symporter Antibody Description

Background
The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells. This is important step in the process of iodide organificaton and the formation of triiodothyronine and thyroxine.
Immunogen
Sodium-Iodide Symporter Antibody was produced in mice by repeated immunizations raised against mannose binding protein hNIS fusion.
Immunogen Type
Other
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Sodium-Iodide Symporter Antibody is suitable for use in WB. Expect a band approximately 97kD, non-glycosylated version at 68kD. Other minor bands associated with hNIS at 160 kDa, and degradation products at ~30 kDa, and ~15kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-Sodium-Iodide Symporter Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Sodium-Iodide Symporter from Human, Mouse, and Rat based on 100% homology with the immunizing sequence. Recognizes both rod and cone alpha subunits. Cross-reactivity with Sodium-Iodide Symporter from other sources has not been determined. Ion Channels research.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
1. Dai G., Levy O., Carrasco N. (1996) Nature. 379(6564): 458-460. 2. Snabik P.A., et al. (1997) Endocrin. 138(8): 3555-3558. 3. Dohan O., et al. (2007) Proc Natl Acad Sci USA. 104(51): 20250-20255.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, chicken
Catalog Number
100-401-401

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry