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Kits  >  Cell Survival Kits
 

CellCountEZ™ Cell Survival Assay Kit

Code

KLD-001
Size

1 Kit
Price

$159.00
Availability

Ships within 2 weeks.
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Schematic representation of the various pathways involved in the cellular interactions of HEDS. HEDS reacts spontaneously with glutathione (GSH) or in a reaction catalyzed by glutathione-S-transferase (GST) to produce mixed disulfide (MESSG) of GSH and mercaptoethanol (ME) (Pathway 1). The mixed disulfide MESSG reacts with GSH and produces ME and GSSG by the catalytic action of glutaredoxin (GRX) (Pathway 2). The glutathione disulfide GSSG reacts with NADPH and produces GSH by the catalytic action of glutathione reductase (GRase) (Pathway 3). The conversion of GSSG to GSH i.e. GSH recycling requires NADPH recycling (NADP+ NADPH) by oxidative pentose phosphate cycle (OPPC) (Pathway 4).
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Synonyms: Toxicity, Proliferation, and Survival Assay, cell proliferation and survival, drug screening, cell viability, cell survival, cellular viability, cellular survival, HEDS assay.

CellCountEZ™ Cell Survival Assay Kit Properties

Description
CellCountEZ™ Cell Survival Assay Kit - KLD-001
Product Type
Kit
Kit Type
Cell Survival Kit
Physical State
n/a
Shipping condition
Wet Ice
Label
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CellCountEZ™ Cell Survival Assay Kit Description

Background
CellCountEZ™ Cell Survival Assay Kit is a tissue culture media-based assay that can measure metabolically active live cells and quantify cell death caused by radiation, chemotherapeutics or toxins. CellCountEZ™ is based on the ability of mammalian cells to rapidly and efficiently convert hydroxyethyl disulfide (HEDS) into mercaptoethanol (ME) through a bioreduction mechanism. Bioconversion of HEDS to ME relies on the activity of the oxidative pentose phosphate cycle (OPPC). CellCountEZ™ measures ME in the extracellular medium without the need for cellular lysis and extraction methods, this preserves the ability to perform other cellular tests in the same culture system. CellCountEZ™ is readily soluble, membrane permeable and converted by live cells intracellularly before transport into the extracellular culture media. CellCountEZ™ offers many advantages that make it superior to common existing methods for quantifying cell growth and survival.
Storage Condition
Store kit at 2-8° C prior to opening. See kit insert for complete instructions.
Application Note
CellCountEZ™ Cell Survival Assay Kit contains enough reagents for 1000 assays using a 96-well plate. This assay gives a linear response for cells (0; 1,000; 5,000; 10,000; 20,000; 40,000) plated in 100 µl growth medium with up to 15% fetal bovine serum in a 96-well plate and measured 20 hours after plating. Drug toxicity could be measured for up to 4 days after treatment with drugs using this assay for 5,000 cells plated in 100 µl growth medium with up to 15% fetal bovine serum in a 96-well plate.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
Li J, Zhang D, Ward KM, Prendergast GC, and Ayene IS. (2012) Hydroxyethyl disulfide as an efficient metabolic assay for cell viability in vitro. Toxicol. In Vitro Jun;26(4):603-12. Ayene IS, Biaglow JE, Kachur AV, Stamato TD and Koch CJ. (2008) Mutation in G6PD gene leads to loss of cellular control of protein glutathionylation: Mechanism and Implication. J. Cell Biochem. 103: 123-135. Ayene IS, Stamato TD, Mauldin SK, Biaglow JE, Tuttle SW, Jenkins SF and Koch CJ (2002) Mutation in the glucose-6-phosphate dehydrogenase gene leads to inactivation of Ku DNA end binding during oxidative stress. J. Biol. Chem. 277: 9929-9935.
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Product Type
Kits;
Catalog Number
KLD-003


Product Type
Kits;
Catalog Number
KLD-002

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry