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Secondary Antibodies  >  Chemiluminescent Western Blot Kits
 

Mouse TrueBlot®: Anti-Mouse Ig Biotin

Rat Monoclonal eB144
Code

13-8817-82
Size

100 µg
Price

$159.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: Biotin, TrueBlot, Biotin TrueBlot ULTRA, Biotin TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting

Mouse TrueBlot®: Anti-Mouse Ig Biotin Properties

Description
Mouse TrueBlot®: Anti-Mouse Ig Biotin - 13-8817-82
Host
Rat
Target Species
Mouse
Known Cross Reactivity
Mouse
Clonality
Monoclonal eB144
Application
Western Blot : 1:2000
Product Type
Component
Kit Type
Immunoprecipitation Kit
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG
Concentration
0.5 mg/mL by UV absorbance at 280 nm
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.09% (w/v) Sodium Azide
Stabilizer
Proprietary
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Mouse TrueBlot®: Anti-Mouse Ig Biotin Description

Background
Mouse IgG TrueBlot® ULTRA is a unique anti-mouse IgG immunoblotting (second step) reagent. Mouse IgG TrueBlot® ULTRA enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western blotting with Mouse IgG TrueBlot® ULTRA, simply substitute the conventional anti-Mouse IgG blotting reagent with Mouse IgG TrueBlot® ULTRA and follow the prescribed protocol for sample preparation and immunoblotting. The Biotin Mouse TrueBlot® ULTRA is the biotinylated format of Mouse IgG TrueBlot® ULTRA. It can be used as a secondary antibody, followed by the use of avidin-peroxidase conjugated (A003-03).
Storage Condition
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Biotin Mouse TrueBlot® ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. Biotin Mouse TrueBlot® ULTRA preferentially detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Biotin Mouse TrueBlot® ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Note: There are two key procedural considerations: 1. When using any TrueBlot® reagent, ensure there is complete reduction of the lysate. 2. Use milk powder for complete and effective blocking of the western blot.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Kong, D., L. Xu, Y. Yu, W. Zhu, D.W. Andrews, Y. Yoon, and T.H. Kuo. 2005. Regulation of Ca2+-induced permeability transition by BCL-2 is antagonized by Drp1 and hFis1. Molecular and Cellular Biochemistry. 272: 187-199. (Rabbit IgG TrueBlot, PubMed) DiPerna, G., J. Stack, A.G. Bowie, A. Boyd, G. Kotwal, Z. Zhang, S. Arvikar, E. Latz, K.A. Fitzgerald, and W.L. Marshall. 2004. Poxvirus protein N1L targets the I-kappaB Kinase complex, inhibits signaling to NF-kappaB by the Tumor Necrosis Factor superfamily of receptors, and inhibits NF-kappaB and IRF3 signaling by Toll-like Receptors. J. Biol. Chem. 279: 36570-36578. (Rabbit IgG TrueBlot, PubMed) Zhang, X., Y. Ozawa, H. Lee, Y. Wen, T. Tan, B. Wadzinski, and E. Seto. 2005. Histone deacetylase 3 (HDAC3) activity is regulated by interaction with protein serine/threonine phosphatase 4. Genes & Development. 19: 827-839. (Rabbit IgG TrueBlot, PubMed) Lehtonen, S., E. Lehtonen, K. Kudlicka, H. Holthöfer, and M.G. Farquhar. 2004. Nephrin Forms a Complex with Adherens Junction Proteins and CASK in Podocytes and in Madin-Darby Canine Kidney Cells Expressing Nephrin. Am J Pathol. 165:923-936. (Rabbit IgG TrueBlot, PubMed) Tyagi A, Agarwal C, Harrison G, Glode LM, Agarwal R. 2004. Silibinin causes cell cycle arrest and apoptosis in human bladder transitional cell carcinoma cells by regulating CDKI-CDK-cyclin cascade, and caspase 3 and PARP cleavages. Carcinogenesis. 25: 1711-20. (Mouse IgG TrueBlot, PubMed)
Specific Reference
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Product Type
Avidin, Biotin, Streptavidin;
Applications
ELISA, WB, IHC
Catalog Number
A003-03

Product Type
Secondary Antibodies;
Applications
WB
Reacts With
Mouse
Catalog Number
13-8817-80

Product Type
Kits;
Applications
WB, IP
Reacts With
Mouse
Catalog Number
00-8811-25

Product Label

 

Description
Biotin TrueBlot® ULTRA for IP/WB
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry