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Primary Antibodies  >  Phospho Specific Antibodies
 

H2AX pS139 Antibody

Rabbit Polyclonal
Code

600-401-H36
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western Blot of Rabbit anti-H2AXpS139 antibody.  
Lane 1:  HeLa Lysate stimulated with adriamycin (24 hr).  
Lane 2:  HeLa Lysate unstimulated.  
Load:  35 µg per lane.
Primary antibody:  H2AXpS139 antibody at 1:1000 for overnight at 4°C.
Secondary antibody:  HRP rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  15.1 kDa, ~18 kDa for H2AXpS139. 
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: H2AFX anitbody, H2A.x, H2AX, histone H2A, gamma-H2AX, H2A histone family member X antibody

H2AX pS139 Antibody Properties

Description
Anti-H2AX pS139 (RABBIT) Antibody - 600-401-H36
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:5,000
Western Blot : 1:1,000
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
GeneName
H2AFX
Download CofA
Lot#  

H2AX pS139 Antibody Description

Background
Histone H2A.X (phospho S139) antibody is ideal for western blotting and ELISA. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA is wrapped around histone-groups, consisting of the core histones H2A, H2B, H3 and H4. As a reaction on DNA Double-strand breaks (DSB) H2AX becomes phosphorylated on serine 139, called gamma-H2AX. ATM, ATR and PRKDCs, kinases of the PI3-family, are responsible for this phosphorylation. The modification can happen accidentally during replication fork collapse, exogenous genotoxic agents, may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes, in the response to ionizing radiation but also during controlled physiological processes such as V(D)J recombination. Mutagenesis experiments have shown that the modification is necessary for the proper formation of ionizing radiation induced foci in response to double strand breaks, but is not required for the recruitment of proteins to the site of DSBs. Gamma-H2AX is a sensitive target for looking at DSBs in cells. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. The role of the phosphorylated form of the histone in DNA repair is under. Anti-H2AX pS139 is ideal for researched interested in Histones, DNA Damage and Repair, and Epigenetics.
Immunogen
Anti-H2AX pS139 purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to the C-terminal region containing serine 139 of human H2AX protein.
Immunogen Type
Peptide
Storage Condition
Store Histone H2A.X (phospho S139) antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-H2AX pS139 antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15 kDa in size corresponding to phosphorylated H2AX protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
Purity/Specifity
H2AX pS139 is directed against the phosphorylated form of human H2AX protein at the pS139 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity with non-phosphorylated human H2AX is minimal by ELISA and western blot. A BLAST analysis was used to suggest 100% cross reactivity with H2AX from human based on the sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Drosophila melanogaster
Catalog Number
600-401-914

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat, chicken, dog, monkey, Xenopus laevis, Arabidopsis thaliana, Caenorhabditis elegans, Fruit fly
Catalog Number
100-401-E81

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry