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SIRT5 Antibody

Rabbit Polyclonal IgG
NCI Collaboration
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Western Blot of Rabbit Anti-SIRT5 antibody.  
Lane A:  human intestine. Lane B:  mouse intestine. Lane C:  rat intestine cell lysate.
Primary antibody: SIRT5 at 2 µg/mL for overnight at 4°C.
Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~34 kDa for SIRT5.
Other band(s): none.
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Number:
200-401-I14
Size:
100 µg
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships within 2 weeks.

SIRT5 Antibody Properties

Description
Anti-SIRT5 (RABBIT) Antibody - 200-401-I14
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human, Mouse, Rat
Clonality
Polyclonal IgG
Application
Western Blot : 1-3 µg/mL
Physical State
Liquid
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
0.5 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.05% (w/v) Sodium Azide
Stabilizer
0.05% BSA
GeneName
SIRT5
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SIRT5 Antibody Description

Background
Anti-SIRT5 Antibody detects human SIRT5. SIRT5 is a human member of a family of proteins called Sirtuins (Sir2-like proteins) and are present in prokaryotes and eukaryotes. All Sir2-like proteins have a sirtuin core domain, which contains a series of sequence motifs conserved in organisms ranging from bacteria to humans. Bacterial, yeast and mammalian sirtuins are able to metabolize NAD and possibly at as mono-ADP-ribosyltransferases. The enzymatic function of sirtuins is not yet completely understood but recent reports of histone-activated Sir2-mediated NAD metabolism and NAD-activated Sir2-mediated histone deacetylation suggest a possible coupled reciprocal activation mechanism involving interactions of Sir2 with NAD and the N epsilon-acetyl-lysine groups of acetylated histones. Anti-SIRT5 Antibody is ideal for investigators involved in Epigenetic , chromatin, or enzyme research.
Synonyms
SIR2L5
Immunogen
SIRT5 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to internal amino acids of human SIRT5 .
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-SIRT5 Antibody is suitable for use in WB. Expect a band approximately 37kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-SIRT5 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-SIRT5 from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-SIRT5 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
1. Imai S, Armstrong CM, Kaeberlein M, Guarente L. Nature 403: 795-800 (2000). 2. Frye RA. Biochem Biophys Res Commun 260: 273-279 (1999). 3. Landry J, Sutton A, Tafrov ST. Heller RC, Stebbins J, et al. Proc Natl Acad Sci USA 97: 5807-5811 (2000). 4. Frye RA. Biochem Biophys Res Commun 273: 793-798 (2000).
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry