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E.coli HCP Control

Other - E.coli
NCI Collaboration
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2D Oriole Stain of E.coli HCP Control Protein.  
Load:  35ug Total HCP.
pH: 5-8.
Exposure:  1.0s.
Stain: Oriole.
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Number:
000-001-J08
Size:
50 µg
Price:
$105.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

E.coli HCP Control Properties

Description
E.coli Host Cell Protein Control Protein (HCP) - 000-001-J08
Host
Other - E.coli
Target Species
Escherichia coli
Known Cross Reactivity
E.coli
Application
ELISA : 1:1000-1:2000
Western Blot : 5 ug
Other Dilution: User Optimized
Product Type
Component
Kit Type
Chemiluminescent Western Blot Kit
Physical State
Liquid (in 1x Loading Buffer)
Shipping Condition
Dry Ice
Label
Concentration
2 mg/ml by modified Lowry assay
Buffer
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Preservative
None
Stabilizer
None
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E.coli HCP Control Description

Background
Host Cell Protein Lysate was prepared by solubilizing mock induced E.coli cells. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
Synonyms
E.coli Host Cell Proteins , HCP antigen, E.coli control antigen
Immunogen Type
Native Protein
Storage Condition
Store Host Cell Proteins control protein at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Application Note
E. coli Host Cell Protein Control Proteins detects over 300 E.coli proteins and is suitable for western blotting and ELISA.
Purity/Specifity
E. coli Host Cell Protein Control Protein was prepared by solubilizing mock induced E.coli cells.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50


Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
E.coli
Catalog Number
100-401-J06

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry