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Kits  >  Chemiluminescent Western Blot Kits
 

Chemiluminescent Western Blot Kit for detection of E.coli HCP

Code

KCAJ07
Size

1 Kit
Price

$449.00
Availability

Ships within 2 weeks.
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2D Western Blot of E.coli HCP Control Protein.  
Load: 35 µg Total HCP.
pH: 5-8.
Exposure:  2.4s.
Primary antibody: Rabbit anti-HCP antibody at 1:50 for overnight at 4°C.
Secondary antibody:  Goat anti-rabbit secondary antibody at 1:10,000 for 30 min at RT.
Block:  MB-070 for 1 hour at RT.
Western Blot of Rabbit anti-HCP antibody.  
Lane 1:  Molecular Weight.  
Lane 2:  Total HCP.  
Load:  10µg per lane.
Primary antibody:  Rabbit anti-HCP cocktail at 1:50 for overnight at 4°C.
Secondary antibody:  Goat anti-rabbit secondary antibody at 1:10,000 for 30 min at RT.
Block:  MB-070 for 1 hour at RT.
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Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: Western Blotting Kit, Chemiluminescent Kit, Peroxidase Kit, Immunoblotting Kit, HCP, Host Cell Protein detection, HCP contaminants detection, ECP, ECP detection, ECP contaminants

Chemiluminescent Western Blot Kit for detection of E.coli HCP Properties

Description
Chemiluminescent Western Blot Kit for detection of E.coli Host Cell Proteins (HCP) - KCAJ07
Known Cross Reactivity
E.coli
Application
Western Blot : User Optimized by Kit Protocol
Other Dilution: User Optimized by Kit Protocol
Product Type
Kit
Kit Type
Chemiluminescent Western Blot Kit
Physical State
n/a
Shipping condition
Wet Ice
Label
Download CofA
Lot#  

Chemiluminescent Western Blot Kit for detection of E.coli HCP Description

Background
Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. The antibody provided in this kit recognizes high and low molecular weight protein fractions from E.coli. This antibody has been validated by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate. The Anti-Host Cell Proteins Antibody provided in this kit detects over 300 E.coli proteins and is suitable for western blotting.
Storage Condition
See kit insert for complete instructions.
Application Note
Use Rockland Immunochemicals’ Chemiluminescent Western Blot Kit for detection of E.coli Host Cell Proteins. This kit is useful for both ''Western blotting'' and ''dot blotting'' methods. After protein separation and transfer, the membrane is probed with Anti-Host Cell Proteins Antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.
Purity/Specifity
This Chemiluminescent Western Blot Kit allows for the detection of E.coli host cell proteins present in samples provided by the user. A positive protein control is included in the kit.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Custom Products;
Catalog Number
CUST92C

Product Type
Custom Products;
Catalog Number
CUST93C

Product Type
Custom Products;
Catalog Number
CUST94C

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry