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Primary Antibodies  >  Cell Cycle Protein Antibodies
 

BrdU Antibody

Mouse Monoclonal 29G6.E8
Code

200-301-H50
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
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Immunofluorescence Microscopy of Mouse Anti-BrdU antibody.  
Tissue:  OCT-embedded E10.5 mouse embryo.  Localization:  40X, section through the developing limb bud.
Fixation:  4% PFA. 
Antigen retrieval:  not required.
Primary antibody:  BrdU antibody at 1:500 in 0.4% PBS+Triton with 1% normal sheep serum overnight at 4°C.
Secondary antibody:  Alexa Fluor 488 Anti-Mouse secondary antibody at 1:200 for 45 min at RT.
Staining:  Double labeled (green/blue) cells represent cells that were actively dividing.
 Bromodeoxyuridine (BrdU) chemical structure representation.
Shipping & Handling Fees Apply.

Shipping:
$20.00 to United States
Handling:
$20.00
Synonyms: Bromodeoxyuridine, 5-bromo-2'-deoxyuridine, BrdU

BrdU Antibody Properties

Description
Anti-BrdU (MOUSE) Monoclonal Antibody - 200-301-H50
Host
Mouse
Known Cross Reactivity
BrdU, IdU, CldU
Clonality
Monoclonal 29G6.E8
Application
ELISA : 1:2000-1:10,000
IF Microscopy : 1:500-1:3000
Flow Cytometry : 1:50-1:100
Western Blot : 1:2000 - 1:5000
Immunohistochemistry: 1:100-1:500
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
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Lot#  

BrdU Antibody Description

Background
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic thymidine nucleoside analog. BrdU is commonly used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and BrdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for BrdU are subsequently used to detect the incorporated BrdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat.
Immunogen
Anti-BrdU mononclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.
Immunogen Type
Other
Storage Condition
Store BrdU Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-BrdU Antibody has been tested as suitable for immunoblot assays. Antibody may be suitable for additional immunoassays including immunofluorescence, flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Antbody will detect incoporated BrdU thymidine analog from replicated cells.
Purity/Specifity
Anti-BrdU Monoclonal Antibody was purified from ascites fluid by Protein A chromatography. This antibody reacts strongly with BrdU. Cross-reactivity is observed with CldU and IdU.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
M. Teta, M. Rankin, S. Long, G. Stein, and J. Kushner. 2007. Growth and Regeneration of Adult ß Cells Does Not Involde Specialized Progenitors. Developmental Cell 12, 817-826, May.
Specific Reference
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Rating
Name
Application
Species
Submitted On
IF Microscopy
Mouse
6/25/2013 02:55:54 PM
Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat
Catalog Number
200-301-400

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, IP, FC
Reacts With
BrdU
Catalog Number
600-401-C29

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, IP, FC
Reacts With
BrdU
Catalog Number
600-401-C29S

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry