14-10_house_marker_thin
 
You may also want to consider these related products.
Anti-ATM Protein Kinase pS1981 (MOUSE) Monoclonal Antibody - 200-301-400
Anti-ATM Protein Kinase pS1981 (MOUSE) Monoclonal Antibody - 200-301-400
Anti-BrdU (RABBIT) Antibody - 600-401-C29S
Anti-BrdU (RABBIT) Antibody - 600-401-C29S
Anti-RABBIT IgG (H&L) (SHEEP) Antibody DyLight™ 488 Conjugated (Min X Bv Ch Gt GP Hs Hu Ms Rt & Sh Serum Proteins) - 611-641-122
Anti-RABBIT IgG (H&L) (SHEEP) Antibody DyLight™ 488 Conjugated (Min X Bv Ch Gt GP Hs Hu Ms Rt & Sh Serum Proteins) - 611-641-122
BOVINE SERUM ALBUMIN 30% Solution - BSA-30
BOVINE SERUM ALBUMIN 30% Solution - BSA-30
Primary Antibodies  >  Cell Cycle Protein Antibodies
 

IdU Antibody

Mouse Monoclonal 32D8.D9
Code

200-301-H51
Size

100 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Western Blot of Anti-IdU Antibody. 
Lane 1: loading control.  Lane 2: FdU.   Lane 3: BrdU.   Lane 4:  IdU.   Lane 5 CldU.   
Load:  20 µg per lane.
Primary antibody:  Anti-IdU antibody at 1:1000 for overnight at 4°C.
Secondary antibody:  IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed:  IdU. 
Other band(s): no cross reactive bands were observed for other nucleoside analogs.
Iododeoxyuridine (IdU) chemical structure representation.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Iododeoxyuridine, 5-iodo-2'-deoxyuridine, IdU

IdU Antibody Properties

Description
Anti-IdU (MOUSE) Monoclonal Antibody - 200-301-H51
Host
Mouse
Known Cross Reactivity
IdU
Clonality
Monoclonal 32D8.D9
Application
ELISA : 1:1000-1:10000
IF Microscopy : User Optimized
FlowCytometry : User Optimized
Western Blot : 1:2000 - 1:5000
Immunochemistry: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
Download CofA
Lot#  

IdU Antibody Description

Background
Iododeoxyuridine (5-Iodo-2'-deoxyuridine, IdU) is a synthetic thymidine nucleoside analog. IdU, like BrdU thymidine analog, is used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and IdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for IdU are subsequently used to detect the incorporated IdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat.
Immunogen
Anti-IdU mononclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.
Immunogen Type
Other
Storage Condition
Store IdU Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-IdU Antibody has been tested as suitable for western blot, ELISA, and immunofluorescence microscopy. This antibody may be suitable for additional immunoassays including flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect to detect incoporated IdU thymidine analog from replicated cells.
Purity/Specifity
Anti-IdU Monoclonal Antibody was purified from ascites fluid by Protein A chromatography. This antibody is specific for IdU. Cross-reactivity is not observed with BrdU, CldU or FdU.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
M. Teta, M. Rankin, S. Long, G. Stein, and J. Kushner. 2007. Growth and Regeneration of Adult ß Cells Does Not Involde Specialized Progenitors. Developmental Cell 12, 817-826, May.
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat
Catalog Number
200-301-400

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, IP, FC
Reacts With
BrdU
Catalog Number
600-401-C29S


Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry