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Primary Antibodies  >  Epigenetic Research Antibodies
 

HDAC2 Antibody

Rabbit Polyclonal
Code

600-401-J19
Size

100 µg
Price

$309.00
Availability

Ships within 2 weeks.
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Western Blot of Rabbit anti-HDAC2 antibody.  
Lane 1:  mouse brain extract.  
Lane 2:  mouse brain extract blocked with peptide.  
Load:  4 µg per lane.
Primary antibody:  HDAC2 antibody at 1µg/mL for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  59 kDa for HDAC2. 
Other band(s): ~45kDa.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: EC 3.5.1.98, HD2, histone deacetylase 2, RPD3, transcriptional regulator homolog RPD3, YAF1, YY1-associated factor 1

HDAC2 Antibody Properties

Description
Anti-HDAC2 (RABBIT) Antibody - 600-401-J19
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human, Mouse, Rat, Primate
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:60,000
IF Microscopy : 1:100-1:500
Western Blot : 1 µg/ml
Immunohistochemistry: 1:100-1:500
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1 mg/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
None
Stabilizer
50% (v/v) Glycerol
GeneName
HDAC2
Download CofA
Lot#  

HDAC2 Antibody Description

Background
Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDAC2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human. Anti-HDAC2 antibodies are ideal for researchers interested in Breast Cancer, Cancer, Chromatin Research, Epigenetics, Histone Deacetylases, and Stem Cell Markers research.
Immunogen
HDAC2 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus region of human HDAC2.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-HDAC2 antibody is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~59 kDa corresponding to the appropriate cell lysate or extract.
Purity/Specifity
Anti-HDAC2 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards HDAC2. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, Rat, and Primate based on 100% sequence homology. Cross-reactivity with HDAC2 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Annemieke J.M. de Ruijter, Albert H. van Gennip, Huib N. Caron, Stephan Kemp, André B.P. van Kuilenburg,. Histone Deacetylases Characterisation of the Classical HDAC FamilyLY, Biochemical Journal Immediate Publication. manuscript BJ20021321. Published on 12 Nov 2002 . [PMID: 12429021]
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human
Catalog Number
600-401-879

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Molecular Biology Reagents;
Applications
ELISA
Catalog Number
MB-064-1000

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry