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Primary Antibodies  >  Neuroscience Antibodies
 

Beta Amyloid pyro E3 Antibody

Rabbit Polyclonal
Code

600-401-J14
Size

50 µg
Price

$309.00
Availability

Ships next business day.
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Immunofluorescence Microscopy of Rabbit Anti-beta Amyloid pyro E3 antibody.  
Tissue:  human brain section.  
Fixation:  0.5% PFA. 
Antigen retrieval:  not required.
Primary antibody:  beta Amyloid pyro E3 antibody  at 5 µg/mL  for 1 h at RT.
Secondary antibody:  Rabbit secondary antibody at 1:10,000 for 45 min at RT.
Localization:  beta Amyloid pyro E3 is nuclear and cytoplasmic.
Staining:  Top: ß Amyloid pyro E3 as as green fluorescent signal, ß Amyloid 3 as yellow signal; and Bottom: ß Amyloid 3 as red signal with co-incubation of ß Amyloid pyro E3 peptide.
Western Blot of Rabbit Anti-beta Amyloid pyro E3 antibody.  
Lane 1:   beta Amyloid 3 peptide  
Lane 2:  beta Amyloid pyro E3 peptide. 
Load:  1 µg per lane.
Primary antibody:  ß Amyloid pyro E3 antibody at 2µg/mL for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  86.9 kDa for beta Amyloid pyro E3 peptide.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Abeta, Amyloid beta, Beta Amyloid peptide, Beta Amyloid Antibody

Beta Amyloid pyro E3 Antibody Properties

Description
Anti-Beta Amyloid pyro E3 (RABBIT) Antibody - 600-401-J14
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human, Bovine, Primate, Sheep
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:60,000
IF Microscopy : 1:100-1:500
Western Blot : 1 µg/ml
Immunochemistry: 1:100-1:500
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
APP
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Lot#  

Beta Amyloid pyro E3 Antibody Description

Background
The cerebral and vascular plaques associated with Alzheimer's disease are mainly composed of Amyloid beta peptides. Beta Amyloid is derived from cleavage of the Amyloid precursor protein and varies in length from 39 to 43 amino acids. Beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides result from cleavage of Amyloid precursor protein after residues 40, 42, and 43, respectively. The cleavage takes place by gamma-secretase during the last Amyloid precursor protein processing step. Beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides are major constituents of the plaques and tangles that occur in Alzheimer's disease. Beta Amyloid antibodies and peptides have been developed as tools for elucidating the biology of Alzheimer's disease. Anti-beta Amyloid [Pyro Glu11] Antibody is ideal for researchers interested in Alzheimers Research, Cell Cycle and Replication, and Neuroscience research.
Immunogen
Beta Amyloid [Pyro Glu3] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-terminus 3-pyro E start point of human beta Amyloid.
Immunogen Type
Peptide
Post Translational Modification
Pyroglutamate
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Beta Amyloid pyro Glu3 antibody is useful for ELISA, Western Blot and Immunostaining. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~86.9kDa corresponding to the appropriate cell lysate or extract.
Purity/Specifity
Anti-Beta Amyloid pyro Glu3 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody contains no reactivity towards the 1-42 ABeta peptide. A BLAST analysis was used to suggest cross-reactivity with Human, Primate, Bovine and Sheep based on 100% sequence homology. It is not reactive with Rodentia. Cross-reactivity with beta Amyloid pyro Glu3 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Schilling S, Lauber T, Schaupp M, Manhart S, Scheel E, Böhm G, Demuth HU. On the seeding and oligomerization of pGlu-amyloid peptides (in vitro). Biochemistry;45(41):12393-9. 2006 Oct 17. [PMID: 17029395] He W, Barrow CJ. The A beta 3-pyroglutamyl and 11-pyroglutamyl peptides found in senile plaque have greater beta-sheet forming and aggregation propensities in vitro than full-length A beta. Biochemistry;38(33):10871-7 . 1999 Aug 17. [PMID: 10451383]
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse
Catalog Number
600-401-253

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Molecular Biology Reagents;
Applications
ELISA
Catalog Number
MB-064-1000

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry