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Primary Antibodies  >  Serum Protein Component Antibodies
 

Bovine Serum Albumin Antibody Peroxidase Conjugated

Rabbit Polyclonal
Code

201-4333-0100
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: ALB antibody, Albumin antibody, Allergen Bos d 6 antibody, BSA antibody, DKFZp779N1935 antibody

Bovine Serum Albumin Antibody Peroxidase Conjugated Properties

Description
Anti-BOVINE ALBUMIN (RABBIT) Antibody Peroxidase Conjugated - 201-4333-0100
Host
Rabbit
Target Species
Bovine
Known Cross Reactivity
bovine
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:20,000
Western Blot : 1:500 - 1:2,000
Immunohistochemistry: 1:250 - 1:1,000
Other Dilution: User Optimized
Species of Origin
Bovine
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Stabilizer
10 mg/ml Polyethylene Glycol (PEG-8000)
Download CofA
Lot#  

Bovine Serum Albumin Antibody Peroxidase Conjugated Description

Background
Bovine serum albumin (also known as BSA or "Fraction V") is a serum albumin protein derived from cows. It is often used as a protein concentration standard. BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes, pipet tips, and other vessels. This protein does not affect other enzymes that do not need it for stabilization. BSA is also commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA. BSA is used because of its stability to increase signal in assays, its lack of effect in many biochemical reactions, and its low cost, since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry. Anti-Bovine albumin antibody is ideal for investigators involved in serum protein component research.
Immunogen
Albumin (Bovine Serum)
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-BOVINE ALBUMIN (RABBIT) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. It is also used as a nutrient in cell and microbial culture. Specific conditions should be optimized by user.
Purity/Specifity
Anti-BOVINE ALBUMIN (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Albumin [Bovine Serum].  Cross reactivity against Albumin from other tissues and species may occur but have not been specifically determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Peters T (1975). Putman FW. ed. The Plasma Proteins. Academic Press. pp. 133–181.
Specific Reference
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Product Type
Proteins and Peptides;
Catalog Number
001-0033

Product Type
Proteins and Peptides;
Catalog Number
001-0133

Product Type
Proteins and Peptides;
Catalog Number
001-0333

Product Type
Proteins and Peptides;
Catalog Number
001-0633

Product Label

 

Description
Peroxidase (Horseradish)
Color
Substrate dependent
Conjugation Reference
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Molecular Weight
40000
Excitation Wavelength
Conjugation Chemistry
Periodate