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Anti-Low Molecular Weight Proteins Host Cell Protein (HCP) Antibody - 100-401-J06
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Primary Antibodies  >  Loading Control Antibodies
 

HMW Host Cell Proteins Antibody

Rabbit Polyclonal
Code

200-401-J05
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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2D Western Blot of anti-E.coli High Molecular Weight Host Cell Protein antibody.  
Load: 35 µg HMW HCP.
Primary antibody: Rabbit anti-HMW-HCP antibody at 1:200 for overnight at 4°C.
Secondary antibody:  Goat anti-rabbit secondary antibody at 1:10,000 for 30 min at RT.
Block:  MB-070 for 1 hour at RT.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Host Cell Protein antibody, HCP antibody, Anti-HCP, Anti-Host Cell Protein, high molecular weight HCP proteins antibody, HMW HCP

HMW Host Cell Proteins Antibody Properties

Description
Anti-High Molecular Weight Proteins Host Cell Protein (HCP) Antibody - 200-401-J05
Host
Rabbit
Target Species
Escherichia coli
Known Cross Reactivity
E.coli
Clonality
Polyclonal
Application
ELISA : 1:1000-1:2000
Western Blot : 1:500 - 1:1000
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.38 mg/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
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Lot#  

HMW Host Cell Proteins Antibody Description

Background
Anti-HMW Host Cell Proteins Antibody recognizes high molecular weight protein fractions from E.coli. This antibody has been anlyzed by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
Immunogen
Anti-HMW Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture high molecular weight (HMW) proteins from E.Coli.
Immunogen Type
Native Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-HMW Host Cell Proteins Antibody detects over 250 E.coli HMW proteins and is suitable for western blotting and ELISA.
Purity/Specifity
HMW Host Cell Protein A purified antibody is directed against high molecular weight E.coli Host Cell Proteins.  The product was purified from monospecific antiserum by Protein A affinity chromatography.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50


Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
E.coli
Catalog Number
100-401-J06

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry