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Primary Antibodies  >  Cancer Research Antibodies
 

CD4 Fluorescein Antibody

Rat Monoclonal GK1.5 IgG2b kappa
Code

200-502-M70
Size

500 µg
Price

$259.00
Availability

Ships within 2 weeks.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Cell Surface Flow Cytometry of Rat anti-MOUSE CD4 antibody Fluorescein conjugated.  
Cells: 10^6 BALB/c mouse splenocytes. 
Stimulation: none.
Antibody:  (GREEN) isotype control antibody; (RED) Fluorescein Anti-CD4 rat secondary antibody using 1 µg.
Shipping & Handling Fees Apply.

Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4 Antibody, L3T4, T4

CD4 Fluorescein Antibody Properties

Description
Anti-CD4 (RAT) Fluorescein Conjugated Monoclonal Antibody - 200-502-M70
Host
Rat
Target Species
Mouse
Known Cross Reactivity
Mouse
Clonality
Monoclonal GK1.5 IgG2b kappa
Application
IF Microscopy : User Optimized
FlowCytometry : 0.1-1 µg/10^6 cells
Other Dilution: Sufficient to run approximately 500 tests
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG2b
FP Value
2-8 moles Fluorescein (FITC) per mole of IgG2b
Concentration
0.5mg/mL by UV absorbance at 280 nm
Buffer
0.01 M Tris Cl, 0.15 M Sodium Chloride, pH 8.0
Preservative
0.05% (w/v) Sodium Azide
Stabilizer
0.05% BSA
GeneName
CD4
Download CofA
Lot#  

CD4 Fluorescein Antibody Description

Background
CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. It is a member of the immunoglobulin superfamily. On T cells, it is the co-receptor for the T cell receptor (TCR) and amplifies the signal generated by the TCR by recruiting the tyrosine kinase lck that is essential for activating many molecules involved in the signaling cascade of an activated T cell. It is an important component of the immune system and is also the cellular receptor for HIV-1. It consists of a cytoplasmic tail, one transmembrane region, and four extracellular domains, D1-D4.  It maps to 12pter-p12 region of human chromosome.
Immunogen
Anti-CD4 Antibody (Monoclonal) was produced by repeated immunizations with Mouse CTL clone V4.
Immunogen Type
Other
Storage Condition
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.
Application Note
Anti-CD4 is useful for Immunofluorescence and Flow Cytometry (Cell Surface). Researchers should determine optimal titers for applications that are not stated.
Purity/Specifity
Fluorescein conjugated CD4 Monoclonal Antibody was Protein G Purified and is directed against mouse CD4. Reactivity is observed against mouse CD4. Cross reactivity with CD4 from other sources has not been tested.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. 2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. 3. Wu L, et al. 1991. J. Exp. Med. 174:1617. 4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. 5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. 6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. 7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. 8. Israelski DM, et al. 1989. J. Immunol. 142:954. 10. Frei K, et al. 1997. J. Exp. Med. 185:2177.
Specific Reference
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Product Type
Proteins and Peptides;
Applications
ELISA, WB
Catalog Number
012-0102

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human, chimpanzee
Catalog Number
200-401-908

Product Type
Secondary Antibodies;
Applications
FLISA, IF, FC
Catalog Number
612-4202

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-10

Product Label

 

Description
Fluorescein (FITC)
Color
Green
Conjugation Reference
Modified from The and Feltkamp, Immunology 18; 865, 1970.
Molecular Weight
390
Excitation Wavelength
495
Conjugation Chemistry
Isothiocyanate