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APP Antibody

Rabbit Polyclonal
NCI Collaboration
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Number:
600-401-G76
Size:
100 µg
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships within 2 weeks.

APP Antibody Properties

Description
Anti-APP (RABBIT) Antibody - 600-401-G76
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Application
Western Blot : 0.5-1 ug/mL
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
1 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
GeneName
Amyloid beta A4 protein
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APP Antibody Description

Background
APP antibody detects the human amyloid A4 protein. Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the brains of all suffers of Alzheimer's disease. Anti-APP is ideal for investigators that are involved in Neuroscience research.
Synonyms
APP (AbNT), APP(AbNT),ABPP
Immunogen
APP Antibody was produced from whole rabbit serum prepared by repeated immunizations with a peptide corresponding to amino acids of human amyloid A4 protein precursor (APP) corresponding to the n-terminus of the 4K Ab peptide generated by b- and g-secretases. The peptide sequences are identical to those of rabbit, pig, bovine, guinea pig, and chicken.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-APP Antibody is suitable for use in E,WB, and IHC. Expect a band approximately ~86.9 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-APP Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with APP with Human, Mouse and Rat based on 100% homology with the immunizing sequence. Cross-reactivity with APP from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Ponte P, Gonzalez-DeWhitt P, Schilling J, Miller J, Hsu D, Greenberg B, Davis K, Wallace W, Lieberburg I, Fuller F. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature 1988;331:525-7. Selkoe D.J. Cell biology of the amyloid β-protein precursor and the mechanism of Alzheimer's disease. Annu Rev Cell Biol 1994;10:373-403 (RD1000)
Specific Reference
N Nagai, Y Ito. (2013) Excessive hydrogen peroxide enhances the attachment of amyloid ß1–42 in the lens epithelium of UPL rats, a hereditary model for cataracts. ScienceDirect Toxicology. Vol 315, 6 Jan 2014, Pages 55-64.
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
100-401-405

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
200-401-984

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse
Catalog Number
600-401-253

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry