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GFP and Epitopes  >  Antibodies to GFP and Antibodies to RFP
 

Anti-mCherry Antibody

Rabbit Polyclonal
Code

600-401-P16
Size

100 µg
Price

$199.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Western Blot of Rabbit anti-mCherry antibody.  
Lane 1:  Rockland mCherry fusion protein (reduced).
Lane 2: Control dsRed reduced.
Lane 3: Control mCherry reduced.
Lane 4: Control BFP reduced.
Lane 5: Control eGFP reduced.
Lane 6:  Rockland mCherry fusion protein (non-reduced).
Lane 7: Control dsRed non-reduced. 
Lane 8: Control mCherry non-reduced.
Lane 9: Control BFP non-reduced.
Lane 10: Control eGFP non-reduced.
Total load ~200 nanograms per lane.
Load: 200ng per lane.
Primary Antibody: Anti-mCherry at 1:5000 for overnight at 4°C.
Secondary antibody: HRP rabbit secondary antibody at 1:10,000 and TMBM-100.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  25.9 kDa, for mCherry and RFP, no reaction to BFP or GFP.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: mCherry monomeric red fluorescent protein, Red Fluorescent Protein (RFP), rDsRed, Discosoma sp. Red Fluorescent Protein, DsRFP, mRFP1, mBanana, mCherry, mHoneydew, mPlum, mOrange, mStrawberry, mTangerine

Anti-mCherry Antibody Properties

Description
Anti-mCherry (RABBIT) Antibody Min X Hu Ms and Rt Serum Proteins - 600-401-P16
Host
Rabbit
Clonality
Polyclonal
Application
ELISA : 1:90,000
IF Microscopy : 1:200 - 1:2,000
Western Blot : 1:2,000 - 1:10,000
Immunochemistry: 1:200 - 1:2,000
Epitope Tag Type
RFP
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
PSC Format
Recombinant Protein
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
GeneName
DsRed
Download CofA
Lot#  

Anti-mCherry Antibody Description

Background
mCherry Antibody is ideal for Western Blotting. Fluorescent proteins such as Discosoma Red Fluorescent Protein (and its varients), and GFP are widely used in research practice. Both commonly serve as a markers for gene expression and protein localization. DsRed was isolated from sea anemone Discosoma sp. mushroom and GFP is originated from Aequorea victoria jellyfish. As DsRed and GFP share only 19% identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a ß-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional steps in the chromophore maturation and obligates tetrameric structure. All mCherry antibodies have been pre-absorbed to eliminate any potential cross-reactivity to human, mouse and rat serum proteins. The antibodies are also confirmed for non-reactivity to GFP protein.
Immunogen
The immunogen is a mCherry mutant varient fusion protein of RFP corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Storage Condition
Store mCherry Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Polyclonal anti-mCherry is designed to detect mCherry, RFP, and its variants. Anti-mCherry (Discosoma sp.) are intended for use in immunological assays including ELISA, western blotting, immunofluorescence, and fluorescence activated cell sorting (FACS). Researchers should determine optimal titers for applications that are not stated. In addition, we performed conjugation of RFP antibodies to either fluorescent dyes, biotin or horseradish peroxidase to further facilitate RFP protein detection and quantification.
Purity/Specifity
mCherry was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Expect reactivity against mCherry, RFP and its variants: tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified mCherry.  No reaction was observed against Human, Mouse or Rat serum proteins.  ELISA was used to confirm specificity at less than 0.1% of target signal.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
GFP and Epitopes;
Catalog Number
000-001-379

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
wt and all variants such as rGFP, eGFP, S65T-GFP, RS-GFP, YFP and EGFP
Catalog Number
600-401-215

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC, IF
Catalog Number
600-401-379

Product Type
GFP and Epitopes;
Applications
FLISA, WB, IF
Catalog Number
600-402-379

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry