AKT1 Human Recombinant Protein Western Blot

Datasheet

AKT1 Human Recombinant Protein

Name: AKT1 Human Recombinant Protein
Brand: Rockland Immunochemicals
Price: 315.0 USD
Availability: InStock

2 References

Item No. 009-001-P21

Size: 10 µg

Applications: WB, SDS-PAGE

Origin: Human

$315.00 /Per Item

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Background

AKT1 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and RAC (Related to A and C kinases). AKT is a key regulator of many signal transduction pathways, and it exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. AKT1 recombinant protein is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.

Expand Background

Specifications for AKT1 Human Recombinant Protein

Product Details

Description: AKT1 Human Recombinant Protein - 009-001-P21

Synonyms: RAC, PKB, AKT, PKB alpha, PRKBA, activated AKT

Species of Origin: Human

Type: Recombinant Protein

Target Details

Gene Name: AKT1 - View All AKT1 Products

Purity/Specificity: Recombinant protein corresponds to amino acids 1 to 480 of mature human AKT1; Akt isoform 1. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. This protein was co-expressed with the p110 kinase domain leading to phosphorylation of key residues T308 and S473. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.

Database Links:

UniProtKB - P31749

GeneID - 207

NCBI - 62241011

Application Details

Tested Applications: SDS-PAGE, WB

Application Note: Human AKT1 recombinant protein has been tested in SDS-Page and western blot and is suitable as a control for polyclonal or monoclonal anti-AKT1 in immunological assays. Akt1 recombinant protein is expected to be phosphorylated and in an active state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Cell Line Data

Induction: None (Control)

Formulation

Physical State: Liquid

Concentration: 1.16mg/ml by UV absorbance at 280 nm

Buffer: 20 mM Tris pH8, 300 mM NaCl with 10% glycerol

Preservative: None

Stabilizer: 10% (v/v) Glycerol

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20°C or colder prior to use. Avoid cycles of freezing and thawing, aliquot and freeze at -20° C or colder. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) .

Expiration: Expiration date is one (1) year from date of receipt.

References (2)

Haapalainen AM et al. (2021). Human CPPED1 belongs to calcineurin‐like metallophosphoesterase superfamily and dephosphorylates PI3K‐AKT pathway component PAK4. J Cell Mol Med.

Applications

WB, IB, PCA

PubMed

Haapalainen et al. (2018). Expression of CPPED1 in human trophoblasts is associated with timing of term birth. Journal of Cellular and Molecular Medicine

Applications

WB, IB, PCA

PubMed

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Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.