Datasheet

MYT1 protein-GST fusion

Name: MYT1 protein-GST fusion
Brand: Rockland Immunochemicals
Availability: OutOfStock

Item No. 009-001-S53

Size: 50 µg

Reactivity: H. sapiens (Human)

Origin: Human

Expressed in: Sf9 cells

This product is discontinued

Background

MYT1 is a member of a family of neural specific, zinc finger-containing DNA-binding proteins which binds to the promoter regions of proteolipid proteins of the central nervous system and plays a role in the developing nervous system. MYT1 of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system(1). The recombinant MYT1 fragments containing either the upstream 2 zinc fingers or the downstream 4 zinc fingers bound the same cis regulatory element in the PLP1 promoter in vitro and DNA binding required Zn(2+), but not other divalent cations (2). MYT1 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cancer, Cell Cycle, and Ser/Thr Kinases research.

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Specifications for MYT1 protein-GST fusion

Product Details

Description: MYT1 recombinant protein-GST fusion protein - 009-001-S53

Synonyms: PKMYT1, FLJ20093, DKFZp547K1610, Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase EC 2.7.11.1, Myt1 kinase

Species of Origin: Human

Expressed in: Sf9 cells

Type: Recombinant Protein

Target Details

Gene Name: PKMYT1

Reactivity: H. sapiens (Human)

Purity/Specificity: Recombinant full-length human MYT1 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >80% by densitometry.

Database Links:

NCBI - NM_004203

Application Details

Application Note: MYT1 Protein is stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. MYT1 Protein is suitable for use in Western Blot. Expect a band approximately ~93kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 0.1 µg/µL

Buffer: See application note.

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiration: Expiration date is one (1) year from date of receipt.

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.