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Standard HCP Reagent Development


Standard HCP Reagent Development

 

The Standard  method is a process is  where the two sets of animals are immunized using high and low molecular weight Host Cell Protein (HCP) extract.  After immunization and primary boosting, antisera is collected.  The developed anti-HCP antibodies are evaluated to determine coverage of potential HMW  and LMW host cell contaminants  . The final HCP antibody product is a pool of anti-HMW and anti-LMW antibodies having broad coverage of the HCP antigens in the final antibody reagent. Coverage is demonstrated by 1D western blot analysis  or 2D western Blot Analysis against the HCP extract.  HCP-detection assay development is available.  To obtain the optimal level of coverage multiple host animals may be utilized. The following species are available; Rabbit, Chicken, Sheep, Goat and Donkey.

 

 

Standard Host Cell Protein

Bioprocessing Related Products

Catalog Number

Applications Size
       
Anti-Bovine Serum Albumin (Rabbit) Antibody

201-4133-0025

ELISA, WB, IHC 25 uL
Anti-Bovine Serum Albumin (Mouse) Monoclonal Antibody (BSA)

201-301-033

ELISA, WB, IHC, IP 500 uL
Anti-Streptavidin (Rabbit) Antibody

200-401-095

ELISA, WB, IHC 100 uL
Anti-Biotin (Mouse) Monoclonal Antibody

200-301-098

ELISA, WB, IHC, IF 100 uL
Anti-6X His Epitope Tag (Mouse) Monoclonal Antibody

200-301-382

ELISA, WB, IP 100 ug
Antibody For The Detection Of Flag™ Conjugated Proteins (Mouse) Monoclonal Antibody

200-301-383

ELISA, WB, IHC 100 ug
Anti-Protein A (Goat) Antibody

100-1177

ELISA, WB, IHC 2 mL
Anti-Protein G (Rabbit) Antibody

100-401-138

ELISA, WB

2 mL

Anti-Bovine Igg (H&L) (Rabbit) Antibody

201-4102

ELISA, WB, IHC 50 mg
       
Host Cell Protein Related Products

Catalog Number

Applications Size
E.Coli Host Cell Protein Control Protein

000-001-J08

ELISA, WB 50 ug
Anti-E.Coli  Host Cell Protein [Total] (Rabbit) Antibody

100-401-J07

ELISA, WB, IHC 100 uL
Anti-E.Coli  High Molecular Weight Fraction Host Cell Protein Antibody

200-401-J05

ELISA, WB 100 ug
Anti-E.Coli Low Molecular Weight Fraction Host Cell Protein Antibody

200-401-J06

ELISA, WB 100 ug
Anti-E.Coli  Host Cell Protein [High And Low Molecular Weight Combined] Antibody

200-401-M61

ELISA, WB 100 ug
Anti-E.Coli  Host Cell Protein Combined Biotin Conjugated Antibody

200-406-M61

ELISA, WB 100 ug
Chemiluminescent Western Blot Kit For Detection Of E.Coli Host Cell Proteins

KCAJ07

WB 1 Kit

 

 

 

Related Links:

 

Host Cell Protein


HCP Assay Development


Cascade Antibody Generation


1D Analysis


2D Analysis