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        Antibody Kits


Introduction

Rockland kits are typically composed of the required antibodies (primary antibodies, secondary antibodies, or types of conjugated antibodies for specific detection), substrates, and any required enzyme for a specific experiment.


Featured Kits


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Rockland offers a number of TrueBlot® products to assist in the accurate detection and isolation of target antigens across a number of assay types. TrueBlot® eliminates heavy/light chain blotting, contamination, and ongoing interference from immunoprecipitation. Increase the clarity of your results with TrueBlot® IP Beads and complete IP/Western Blot kits from goat, mouse, rabbit or sheep.



Assay Kit Benefits

 
Assay kits are a great way to cut costs, ensure all components for specific tests are purchased at the same time, and to increase accuracy. The components of Rockland's kits are specifically designed to work effectively with one another, ensuring accurate and timely results. We take tremendous care in assembling the types of kits researchers are looking for, including kits for Western Blot analysis, ELISA, Immunohistochemistry, FLISA, and many others.

Purchasing a kit from Rockland is fast and easy, requiring just a few specific inputs based on your application. From there, our scientists can help you choose the kit that best fits your needs, or even help in the development of custom products to suit your project. Our process is based on flexibility and we treat your projects as if they are our own.

Specific benefits of kits over purchasing separate components include:

    Fast Results – Kits can often directly or indirectly cut analysis time as they require less upfront setup and optimization.

    High Sensitivity – Our kits maintain the Rockland standard for sensitivity, making them the same as or better than that of classical procedures.

    Reproducible Results – Like all Rockland products, kits provide highly reproducible and accurate results.

    Economical – Purchasing in a kit saves on a variety of shipping and packaging costs, and ensures that all materials needed for analysis are available at the time of purchase.
 


Antibody Pathway Kits

 
Rockland provides antibody pathway kits that consist of 3-5 trial size antibodies, offering researchers a cost effective and convenient method to examine numerous aspects of cellular signaling. Each kit contains several sample size antibodies, focused on the protein, process, or pathway of interest. Additionally, the required secondary antibodies and reagents make detection and final analysis accurate and reproducible on a number of scales. Each kit comes with all of the components required to perform up to four separate analyses.





Reliable Results

 
Typical results from Rockland antibody kits mirror that of our individual products. We are focused on quality and strive to ensure accuracy as well as consistency in produced results. Analytical results for a few of our kits are pictured below, but contact us for more information on expected outcomes for specific analyses.



Chemiluminescent Western Blot Kit shows super sensitive signal     Publication quality western blot results     Transcription factor assay absorbance  
         


 
   

 



Kit Applications

 


Protect your results with Rockland kits. Compromise elsewhere.
For more information or a custom quote, contact us today!  


 
 

TrueBlot® Mouse
Western Blot kit

 


 

The Mouse TrueBlot® Western Blot Kit contains the critical supporting reagents, buffers, and substrates for Akt immunoprecipitation and Western blotting of samples using TrueBlot second step immunoblotting reagents in conjunction with your own primary IP antibody and primary (Mouse IgG) Western blotting antibody. TrueBlot technology enables unhindered detection of protein bands of interest which would otherwise be obscured by the presence of reduced and denatured heavy and light chain immunoglobulin in the blot (as detected by the conventional immunoblotting HRP anti-mouse IgG reagent). Mouse IgG TrueBlot® ULTRA is the unique horseradish peroxidase conjugated anti-mouse IgG immunoblotting second step reagent which enables detection of Akt immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your usual HRP anti-mouse IgG immunoblotting second step reagent. It is easy to generate publication-quality Akt IP Western Blot data with Mouse IgG TrueBlot® ULTRA.

 


Mouse IgG TrueBlot ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in Akt IP Western Blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.

  

IP / Western Blotting comparison of TrueBlot and conventional reagents