Secondary Antibodies: Buy 1, Get 1 Free
Biotin Beta Galactosidase
Streptavidin DyLight™ 488
Avidin, a tetrameric biotin binding protein that shows high sequence homology in birds, reptiles and amphibians, is deposited in the egg white. Hen egg white avidin is a tetrameric protein composed of four identical subunits, each with the ability to bind biotin with high affinity and specificity (Kd ~ 1015 M). In biotechnology, the functional consequence of tetrameric biotin binding is signal amplification. Biotin-avidin bridging is a great way to increase signal strength while maintaining specificity. Avidin is a glycoprotein with a molecular weight of approximately 62.4 kDa.
Avidin Peroxidase Conjugate for western blotting
Avidin Fluorescein Conjugate for immunofluorescence miscroscopy
Avidin Alkaline Phosphatase Conjugate for ELISA
In biotechnology biotin is most often conjugated to antibodies. Other proteins, including enzymes, are often coupled to biotin. By exploiting the tetravalent binding of avidin, a biotin-avidin-biotin complex, can be formed. By example, a biotinylated antibody can be indirectly linked to a biotin conjugated beta Galactosidase through avidin resulting in signal amplification as an intended consequence of biotin-avidin bridging.
Biotin Peroxidase Conjugate for western blotting
Biotin Beta Galactosidase Conjugate for molecular biology
Biotin Glucose Oxidase Conjugate for ELISA
Streptavidin has identical biotin binding properties compared with avidin, but lacks the glycoprotein portion of the molecule and therefore shows less non-specific binding. Streptavidin is a slightly smaller molecule with a molecular weight of approximately 53.6 kDa. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Rockland offers streptavidin conjugated to a wide variety of enzymes and fluorochromes, including ATTO, DyLight™ and Cy™ dyes.
Streptavidin Peroxidase Conjugate for western blotting
Streptavidin Cy5™ for immunofluorescence microscopy
Streptavidin DyLight™ 800 Conjugated for fluorescent western blotting
Rockland Immunochemicals Inc.Gilbertsville, PA 19525E-mail: email@example.comPhone: 800.656.7625