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Cell signaling governs basic cellular activities and coordinates cell actions through a complex coordination of responses to cellular microenvironment. The disruption or errors found within this communication chain gives rise to various diseases and cancers. By understanding cell signaling, diseases may be treated more effectively and cures created.
The complex interaction of the activated receptor with other proteins inside the cell before the ultimate physiological effect of the ligand on the cell's behavior is produced is called signal transduction or cell signaling. Numerous cell signaling pathways exist, including Akt, NF-kB, and Notch signaling. Rockland produces antibodies that assist in research for a variety of these pathways.
Akt , also known as Protein Kinase B (PKB), is a serine/threonine-specific protein kinase that plays an integral role in mediating signals for cell growth, survival, cell-cycle progression, differentiation, transcription, translation, and glucose metabolism. Akt is one of the most actively studied kinase pathways in the basic research and drug development arenas. Recent advances in Akt signaling have focused on understanding even more cellular processes and identifying cellular substrates that are physiologically relevant in vivo. These efforts have uncovered important roles for Akt regulation in cancer research, neuroscience, and disease prevention.
Nuclear Factor kappa-light-chain-enhancer of activated B cells or NF-κB is a protein complex that controls the transcription of DNA. It is found in almost all animal cells where they adjust a broad range of biological processes including innate and adaptive immunity, inflammation, stress responses, B cell development, and lymphoid organogenesis. Misregulated NF-κB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. Many natural products promoted as having anti-cancer and anti-inflammatory activity have been shown to inhibit NF-κB.
The Notch signaling pathway is a fundamental evolutionarily conserved signaling system allowing neighboring cells to communicate and perform their proper developmental role. Notch proteins mediate critically important cellular functions through direct cell-cell contact. Notch signaling involves gene regulation mechanisms that control multiple cell differentiation processes during embryonic and adult life.
STAT signaling may hold the key to some immune deficiency research and various types of cancers. By studying the autophosphorylation behavior of this pathway, and the influence of STAT proteins in other pathways, researchers gain valuable insight into understanding how this essential signaling system interacts with cellular functions. Rockland develops a variety of antibodies against this pathway that have proven instrumental in continuing the ongoing research within the cancer and immunology fields.
Other areas in which Rockland is helping promote active research for cell signaling analysis include P53, and C-met.
The Mouse TrueBlot® Western Blot Kit contains the critical supporting reagents, buffers, and substrates for AKT immunoprecipitation and Western blotting of samples using TrueBlot second step immunoblotting reagents in conjunction with your own primary IP antibody and primary (Mouse IgG) Western blotting antibody. TrueBlot technology enables unhindered detection of protein bands of interest which would otherwise be obscured by the presence of reduced and denatured heavy and light chain immunoglobulin in the blot (as detected by the conventional immunoblotting HRP anti-mouse IgG reagent). Mouse IgG TrueBlot® ULTRA is the unique horseradish peroxidase conjugated anti-mouse IgG immunoblotting second step reagent which enables detection of AKT immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your usual HRP anti-mouse IgG immunoblotting second step reagent. It is easy to generate publication-quality AKT IP Western Blot data with Mouse IgG TrueBlot® ULTRA.
Mouse IgG TrueBlot ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in AKT IP Western Blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.
Rockland Immunochemicals Inc.Gilbertsville, PA 19525E-mail: email@example.comPhone: 800.656.7625