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Cell Viability Assay and Antibodies


Cell Survival Assays and Antibodies

Intracellular ThiolEZ™ Cell Survival Assay

Anti-BrdU Antibodies

Oxphos™ Cell Survival Assay

CellCountEZ™ Cell Survival Assay Kit

RadChemDosi™ Cell Survival Assay

 

  

 

 

   
                              
 

  

Cell Survival Assays and Antibodies 

  
Cell Viability assays and BrdU detection antibodies are ideal for the determination of proliferation, cell cycle, or cell survival, based on a total cell population. Cell viability assesses healthy cells in a sample, with no distinction between dividing or quiescent cells. An increase in cell viability indicates cell growth, while a decrease in viability can generally be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions.  
 
 

View All Cell Viability Antibodies

 

  

Anti-BrdU Antibodies 

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic thymidine nucleoside analog. BrdU is commonly used to allow the detection of growing or proliferating cells in living tissues.

 

Immunocytochemical detection of BrdU incorporated into DNA is a powerful tool to study cytokinetics of normal neoplastic cells. When cells are cultured with medium containing BrdU, this pyrimidine analog is incorporated in place of thymidine into the replicating DNA of proliferating cells. In vitro or in vivo labeling of tumor cells with BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. The amount of BrdU incorporated into the newly synthesized DNA of S-phase cells is a direct indication of cell proliferation. Also, dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU / DNA FACS / Flow Cytometry analysis.
 
 

  

 

View all BrdU Antibodies

 
  

 

  

CellCountEZ™ Cell Survival Assay Kit 

The CellCountEZ cell survival assay kit offers many advantages that make it superior to common existing methods for quantifying cell growth and survival. This Cell Viability Assay is a tissue culture media-based assay that can measure and is based on the ability of mammalian cells to rapidly and efficiently convert hydroxyethyl disulfide (HEDS) into mercaptoethanol (ME) through a biorreduction mechanism. Bioconversion of HEDS to ME relies on the activity of the oxidative pentose phosphate cycle (OPPC). CellCountEZ measures ME in the extracellular medium without the need for cellular lysis and extraction methods. This preserves the ability to perform other cellular tests in the same culture system. CellCountEZ™ is readily soluble, membrane permeable and converted by live cells intracellularly, before transport into the extracellular culture media.
 
  • Consistent results from tests on the same sample
  • Optical Density Range is up to 3.0 per 40,000 cells
  • Consistent reproducibilty of results means the test is more reliable, resulting in a reduced need for follow-on testing
  • CellCountEZ™ and other reagents in kit are stable for at least six months.
  • No mixing with electron acceptor/donor
  • Measured by standard microplate reader
  • Sample reusable when the cells are washed off of the reagents

 

 

   

CellCount EZ™

 

Best of XTT & WST   

(Tetrazolium Salts)  

         

 

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Quantitate Cell Proliferation, Ideal for Drug Screening and Toxicology, Readily Soluable and Membrane Permeable
 

 

 

CellCountEZ Figure

 

  


 

 

Intracellular ThiolEZ™ Cell Survival Assay

  

Glutathione is a natural antioxidant and a major thiol that limits cellular damage in healthy tissues during oxidative stress. It is also centrally involved in repairing damage induced by cancer drugs and radiation and in the detoxification of several commonly used cancer chemotherapeutic drugs. The Intracellular ThiolEZ cell survival assay kit measures total intracellular thiols including glutathione and cysteine in a simple and user friendly assay. If may have multiple applications for research in aging, oxidative stress, antioxidant screening, chemotherapy response and toxicology.
 
Contains enough reagents for 100 assays and measure Total Intracellular thiols including glutathione and cysteine.
 
   

ThiolEZ™

 

Best of totalthiol detection

(HPLC, biochemical assays)

         

 

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TotalThiolEZ Figure

 

 

 


 
 

Oxphos™ Cell Survival Assay

 

The Oxphos cell survival assay measures the "antioxidant capacity" of cells that requires glutathione. The reagents used are unique in measuring several cytosolic glutathione dependent antioxidant pathways in a single metabolic assay. Oxphos offers many advantages that make it superior and unique to existing methods since it is the only assay that quantifies glutathione function / recycling / glucose dependent antioxidant capacity of all mammalian cells and whole blood.  
 
Obtain Linear Response for Mammalian Cells, Antioxidant Screening, Chemotherapy Response and Toxicology. 
 
 

Oxphos™

   

 

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Oxphos figure

 

 

 

 

RadChemDosi™ Cell Survival Assay  

 

RadChemDosi is a chemical based Cell Viability Assay that can measure the dose rate of ionizing radiation. RadChemDosi measures the dose rate for ionizing radiation by monitoring ionizing radiation to induce changes in certain chemicals. RadChemDosi cell survival assay is ideal for blood irradiators where it can be used for periodic calibration and to determine the dose given to blood by simultaneously irradiating RadChemDosi in small disposable tubes outside the bag.
 
 
Chemical Based Viability Assay and sensitive to measure the dose rate as low as 2.5 Gy/min.
 
   

RadChemDosi™

 

 Competitor Product

         

 

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 $3,600

 

 

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  TrueBlot IP/Western Blot Mouse Rabbit Goat

 

 

 

 Flow Cytometry Protocol Button


      

 

 New Lab Set Up 

FOR LIFE SCIENCE RESEARCH USE ONLY