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Rockland has a number of custom services to meet a variety of research needs. Let Rockland aid you in the expression of your antigen of choice. Our expertise in molecular biology will customize the process, allowing you to proceed from identified gene to target protein without complications. Gene-specific forward and reverse primer design inclusive of identified restriction enzyme sites, followed by PCR DNA amplification, facilitate the cloning of your gene into a cloning vector prior to DNA sequence confirmation.
Rockland’s suite of inducible expression vectors enable the expression of fusion proteins (MBP, GST) or epitope-tag proteins (His6, Flag) in Escherichia coli followed by affinity purification of the harvested protein. Recombinant protein production is offered on a per liter culture basis and can be scaled up according to your requirements.
Rockland’s insect cell-derived protein expression services accommodate post-translational modified recombinant proteins. Generation of Bacmid DNA incorporating the gene of interest facilitates the production of infectious baculovirus for transfection of Sf9, Sf22 or High5 cells. Recombinant protein production is offered on a per liter culture basis and can be scaled up according to your research-related requirements.
Rockland can assist you in the chemical synthesis of your target analytes; chemical compounds such as drugs, drug metabolites, mycotoxins, steroids and other bioactive substances (haptens). Commonly these low molecular weight (MW<500) compounds are non-immunogenic; however; Rockland’s proven expertise will guide you in the generation of highly immunogenic hapten-protein conjugates for antibody development purposes. Our chemistry approach can be employed to couple haptens containing reactive functional groups to carrier proteins such as BSA, KLH or OVA. Should your selected analyte not contain any reactive functional group; Rockland’s staff can synthesize an analyte analog containing a reactive group suitable for coupling purposes.
Hapten-protein conjugates for immunization purposes for the development of small molecule-specific antibodies
Chemical modification of small molecule analyte showing installation of functional group (COOH) for preparation of hapten-protein conjugate.
Rockland Immunochemicals Inc.Gilbertsville, PA 19525E-mail: firstname.lastname@example.orgPhone: 800.656.7625