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FLAG™ tag is a short peptide sequence (DYKDDDDK) that is easily and specifically recognized by the anti-FLAG tag antibody. Because of its small size, FLAG tag does not affect the biochemical properties, transport, or post-translational modifications of the tagged protein or its interaction with other proteins. These properties give scientists valuable tools to study protein expression, modification, localization, transport and the ability to obtain a high yield of purified proteins. Indeed, antibody anti-FLAG tag can detect its target in western blot, immunofluorescence, immunohistochemistry and immunoprecipitation with high specificity. When combined with a resin, anti-FLAG allows an easy, fast and high-yield recovery of homogeneous DYKDDDDK-tagged recombinant proteins.
Antibody for the detection of FLAG™conjugated proteins (RABBIT)
Antibody for the detection of FLAG™
(MOUSE) Monoclonal Antibody
Anti-DYKDDDDK Affinity Gel
The strong and specific interaction between Rockland anti-Flag antibody and its targets is ideal for performing western blot and immunofluorescence assays with high signal detection and low signal noise. These properties limit false negative results in western blot assays and photo-bleaching in immunofluorescence studies.The anti-DYKDDDDK Affinity Gel is a purified mouse IgG2a monoclonal antibody that is highly specific to the DYKDDDDK peptide coupled to activated agarose. The Rockland anti-flag tag is suited for optimal scalable purification and the immunoprecipitation of FLAG®-tagged fusion proteins. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin under acidic conditions or by competition with the FLAG™ peptide. The purification of FLAG-tagged fusion proteins is performed with mild, non-denaturing buffers. Therefore, FLAG-tagged proteins are recovered without loss of function or loss of activity of the protein.
Antibody for the detection of FLAG conjugated proteins (RABBIT)
Antibody IRDye 800CW conjugated
Antibody Peroxidase conjugated
Antibody for the detection of FLAG conjugated proteins (MOUSE)
Monoclonal Antibody – Biotin conjugated
Immunoprecipitation Kit: DYKDDDDK (FLAG)
Rockland Immunochemicals Inc.Gilbertsville, PA 19525E-mail: email@example.comPhone: 800.656.7625