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FLAG™ Tag


FLAG™ Tag Overview

 

FLAG™ tag is a short peptide sequence (DYKDDDDK) that is easily and specifically recognized by the anti-FLAG tag antibody.  Because of its small size, FLAG tag does not affect the biochemical properties, transport, or post-translational modifications of the tagged protein or its interaction with other proteins. These properties give scientists valuable tools to study protein expression, modification, localization, transport and the ability to obtain a high yield of purified proteins. Indeed, antibody anti-FLAG tag can detect its target in western blot, immunofluorescence, immunohistochemistry and immunoprecipitation with high specificity. When combined with a resin, anti-FLAG allows an easy, fast and high-yield recovery of homogeneous DYKDDDDK-tagged recombinant proteins. 

 

 

 
 

dykddddk

 


 

 

The Rockland Anti-FLAG® Tag Products Advantage



The strong and specific interaction between Rockland anti-Flag antibody and its targets is ideal for performing western blot and immunofluorescence assays with high signal detection and low signal noise. These properties limit false negative results in western blot assays and photo-bleaching in immunofluorescence studies.

The anti-DYKDDDDK Affinity Gel is a purified mouse IgG2a monoclonal antibody that is highly specific to the DYKDDDDK peptide coupled to activated agarose. The Rockland anti-flag tag is suited for optimal scalable purification and the immunoprecipitation of FLAG®-tagged fusion proteins. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin under acidic conditions or by competition with the FLAG™ peptide. The purification of FLAG-tagged fusion proteins is performed with mild, non-denaturing buffers. Therefore, FLAG-tagged proteins are recovered without loss of function or loss of activity of the protein.

 

 

Product Name Catalog # Applications Size

Antibody for the detection of FLAG conjugated proteins (RABBIT)

600-401-383   E, WB, IHC 250 μg

Antibody for the detection of FLAG conjugated proteins (RABBIT)

Antibody Dylight 800 conjugated

600-445-383   FLISA, WB, IHC 100 μg

Antibody for the detection of FLAG conjugated proteins (RABBIT)

Antibody Peroxidase conjugated

600-403-383   E, WB, IHC 100 μg

Antibody for the detection of FLAG conjugated proteins (MOUSE)

Monoclonal Antibody

200-301-383 E, WB, IHC 100 μg

Antibody for the detection of FLAG conjugated proteins (MOUSE)

Monoclonal Antibody – Biotin conjugated

200-306-383   E, WB, IHC 100 μg

Anti-DYKDDDDK Affinity Gel

200-350-383   IP 1 mL

Immunoprecipitation Kit: DYKDDDDK (FLAG)

KBA-319-383 IP 1 kit

 

 

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

 

Reference Rockland Bucket

 

 

 

 

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