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Seeing is believing. You can literally see the quality of Rockland antibodies using a variety of techniques including immunofluorescence microscopy. Visual evidence of proper target localization and low background staining is immediately apparent when cells are screened after staining. Protect your experiments with Rockland antibodies. Compromise elsewhere.
Visualizing biological samples using a fluorescent microscope and antibodies conjugated to fluorescent labels is a powerful technique to localize structures within tissues, cells or subcellular compartments. This technique uses the specificity of antibodies to their antigen to target fluorescent dyes to specific targets within a cell, and therefore allows visualization of the distribution of the target molecule through the sample. Immunofluorescence microscopy is a widely used example of immunostaining and is a form of immunohistochemistry based on the use of fluorophores to visualize the location of bound antibodies.
Immunofluorescence can be used on tissue sections, cultured cells or individual cells that are fixed by a variety of methods. Antibodies can be used in this method to analyze the distribution of proteins, glycoproteins and other antigen targets including small biological and non-biological molecules.
Immunofluoresence microscopy can be used in several microscope designs for analysis of immunofluorescence samples. The simplest is the epifluorescence microscope. While confocal microscope is widely used, newer designs of super resolution microscopes, such as STED (Stimulated Emission Depletion) microscopy and others, allow for nanoscopy and are capable of much higher resolution.
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There are two classes of immunofluorescence techniques, primary (or direct) and secondary (or indirect).
Rockland conjugates a select group of secondary antibodies to a new generation of patented fluorescent markers from ATTO-TEC including ATTO 425, ATTO 488, ATTO 532, ATTO 550, ATTO 594, ATTO 647N and ATTO 655. The antibodies are designed for primary antibody detection and multiplex, multi-color analysis. ATTO-TEC fluorochrome conjugates offer strong absorption (high extinction coefficient), high fluorescence quantum yield and superior high photostability. These conjugates are ideal for various immunofluorescence based assays including immunofluorescence microscopy, FLISA, STED microscopy, fluorescent western blotting, time resolved spectroscopy and FRET (Fluorescence Resonance Energy Transfer) applications as well as single-molecule detection (SMD).
CyDye® conjugated antibodies (i.e. Cy2, Cy3, Cy5) are popular choices for fluorescent labeling in applications such as fluorescence microscopy, flow cytometry and fluorescent immunoassays. CyDyes are excellent alternatives to most other fluorescent dyes as they are brighter and offer greater photostability. Depending on the specific CyDye, they may also produce less background and may be less sensitive to pH.
DyLight™ conjugated antibodies (i.e. DyLight 405, DyLight 488, DyLight 549, DyLight 649, DyLight 680 and DyLight 800) are high-performance fluorescent conjugates for use as secondary antibody assays such as fluorescence microscopy, flow cytometry, western blotting, ELISA, high-content screening, multiplex assaysand other array platforms. The antibodies are offered as highly functional conjugates with bright emission spectra that match the principal output wavelengths of common fluorescence instrumentation. DyLight Conjugates exhibit higher fluorescence intensity and photostability than many other dye conjugates.
IRDye® infrared conjugated secondary antibodies (i.e. IRDye800, IRDye800CW, IRDye700 Antibody Conjugates) are ofte ncross-adsorbed for multiplex detection and provide high specificity for assays such as fluorescence microscopy, flow cytometry, western blotting, ELISA, multiplex assays and other array platforms. These antibody conjugates are optimized for high sensitivity andexcellent signal-to-noise performance on LI-COR Odyssey® imaging systems and other detection platforms.
Browse all our fluorescent labeled primary antibodies
Browse all our fluorescent labeled secondary antibodies
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic thymidine nucleoside analog. BrdU is commonly used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and BrdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for BrdU are subsequently used to detect the incorporated BrdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat.
Rockland Immunochemicals Inc.Gilbertsville, PA 19525E-mail: email@example.comPhone: 800.656.7625