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Fluorescent-labeled antibodies can be used to detect target proteins in fluorescent western blots, other immunoblots and FLISA assays, as well as, analyze the distribution of proteins, glycoproteins and other antigen targets in immunofluorescent microscopy and immunohistochemistry. This provides considerable insight into the movement and utilization of antibodies throughout various biological systems. Recently, some fluorophores have shown to have additional uses in fluorescence-based flow cytometry.
Primary, or direct, immunofluorescence uses a single antibody conjugated directly to a fluorescent dye. Secondary, or indirect, immunofluorescence uses two antibodies; a primary antibody which recognizes the target biomolecule and binds to it and a secondary antibody conjugated to a fluorescent dye, which recognizes and binds to the primary antibody and indirectly localizes the target for detection.
Goat IgG (H&L) Antibody ATTO 425 Conjugated Pre-Adsorbed Rabbit Polyclonal
Rabbit IgG (H&L) Antibody DyLight™ 488
Conjugated Pre-Adsorbed Goat Polyclonal
GST Antibody Fluorescein Conjugated Rabbit Polyclonal
Fluorescent dyes are optimized for excitation and detection in the visible light spectrum, 400 nm - 680 nm.
Rockland conjugates a broad group of secondary antibodies to many of the classic fluorescent markers including fluorescein, Rhodamine, Texas Red, CyDyes™ and Phycoerythrin (RPE). All of the conjugates are ideal for various immunofluorescence based assays including fluorescent western blotting, immunofluorescence microscopy, FLISA, and more. Rockland also produces many next generation fluorochrome dyes designed for detection of primary antibodies in multiplex, multi-color analysis. Next generation fluorochrome conjugates (Atto-tec dyes, DyLight™ dyes) offer superior absorption (high extinction coefficient), high fluorescence quantum yield, and superior high photostability.
Western Blot (Immunoblot) Protocol
Immunofluorescence Microscopy Protocol
Red Fluorescent Protein Antibodies
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Rockland Immunochemicals Inc.Gilbertsville, PA 19525E-mail: firstname.lastname@example.orgPhone: 800.656.7625