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Fluorescent Western Blot Buffers


Blocking Buffer for Fluorescent Western Blotting

 

Rockland produces blocking buffers that are highly effective as blocking reagent for all western blot applications including fluorescent, chemiluminescent and 2-dimensional western blot. Fluorescent blocking buffer is specially formulated to preserve the fluorescence from fluorochrome dye conjugated secondary antibodies and also provide an ultra-low background compared to other blocking buffers. Our buffers work with many fluorescent secondary antibody conjugates including, but not limited to, FITC, IRDye, Atto dye, and DyLight dye.
 
  

 

 

 

The Rockland Advantage: Blocking Buffer for Fluorescent Western Blotting

 

Rockland offers blocking buffers for use with Fluorescence based detection systems using the near-UV (blue) through near-IR (red) spectrum and has been validated for producing robust western blots on systems including the Li-Cor Odyssey®, Bio-Rad Chemi-Doc™ MP, UVP BioSpectrum®, Protein Simple Fluorochem and GE Healthcare Typhoon Systems.
Our MB-070 buffer has been demonstrated as highly effective in:
  • Fluorescent western blot
  • Chemiluminescent blots
  • 2-D western blots
  • Multiplex western blot applications
  • Dot blot immunoassays
Blocking Buffer for Fluorescent Western Blotting is exceptionally good in standard chemiluminescent blotting applications, and in head to head comparisons with other blocking reagents is demonstrated to be superior for 2D western blot experiments. 

 


    

2D-WB

 

HRP-anti-mouse-IgG

 

2-D Western Blotting

 

 

 

610-403-C46 HRP

conjugated anti-mouse IgG

   

 

  Available Blocking Buffer for Fluorescent Western Blotting Products

  Catalog #

  Size

 Blocking Buffer for Fluorescent Western Blotting

  MB-070

 500 mL

 Blocking Buffer for Fluorescent Western Blotting

  MB-070S

 125 mL

 Blocking Buffer for Fluorescent Western Blotting 3-PACK (3 x 500 ml)

  MB-070-003

 500 mL each

 Blocking Buffer (2X) for Fluorescent Western Blotting

  MB-070-0050

 50 mL

 Blocking Buffer for Fluorescent Western Blotting 10-PACK (10 x 500 ml)

  MB-070-010

 500 mL each

 Blocking Buffer for Fluorescent Western Blotting

 THIMERISOL FREE 10-PACK (10 x 500 ml)

 

  MB-070-010TF 

 

 

 500 mL each

 

 


 

Related Links

 

 

Reference Rockland Bucket

 

   

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