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        Gel Electrophoresis Markers


Rockland’s Gel Electrophoresis Markers


Rockland’s Gel Electrophoresis Marker (GEM) product line offers a variety of prestained protein standards and DNA ladders for electrophoresis applications. Protein standards suitable for SDS- PAGE and western blot analysis, and DNA ladders for use in agarose gels, are provided as ready-to-use markers. All GEM products display an array of bands to eliminate molecular weight size ambiguity, while reducing the need for hands-on work. These broad range markers are ideal for use in a variety of gels with a variety of running buffers.

The GEM product line is designed for ease, convenience and reliability. The ready-to-use format, combined with their stability at room temperature for 6 months, dramatically reduces the time required to prepare gel electrophoresis applications. Less work, more results.

 

 

 

 

 

Rockland GEM Broad Range Prestained Protein Standards


Rockland GEM Broad Range Prestained Protein Standards are available in dual-colored and tri-colored formats for easy readability. Stable at room temperature for up to 6 months, the standards allow for stress-free observation of protein separation during SDS-polyacrylamide gel electrophoresis (SDS-PAGE). They are suitable for the detection of protein size after Western blot transfer onto membranes (PVDF, nylon, or nitrocellulose).

 


Ruby Prestained Protein Standard 10-175kDa

Sapphire Prestained Protein Standard 10-180kDa

Opal Prestained Protein Standard 10-180kDa

Opal Prestained Protein Standard 10-245kDa

Opal Prestained Protein Standard 3.5-245kDa

 

 

Rockland GEM Broad Range DNA Ladders


Diamond Ready-to-Use DNA Ladders contain a unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield various fragments, suitable for use as molecular weight standards for agarose gel electrophoresis applications.


Diamond Ready-to-Use DNA Ladder 100-1500bp

Diamond Ready-to-Use DNA Ladder 100-3000bp

Diamond Ready-to-Use DNA Ladder 250-10Kbp

Diamond Ready-to-Use DNA Ladder 100-10Kbp

 

Diamond Ready-to-Use DNA Ladder 50-150bp

 

Diamond Ready-to-Use DNA Ladder 250-25kbp

 

 

Related Products:

 

Loading Controls
Blocking Buffers
TrueBlot
Secondary Antibody
Western Blotting
  

 

Related Protocols:


SDS-PAGE
Western Blot
 

 

 

TrueBlot® Mouse
Western Blot kit

 



  

The Mouse TrueBlot® Western Blot Kit contains the critical supporting reagents, buffers, and substrates for Akt immunoprecipitation and Western blotting of samples using TrueBlot second step immunoblotting reagents in conjunction with your own primary IP antibody and primary (Mouse IgG) Western blotting antibody. TrueBlot technology enables unhindered detection of protein bands of interest which would otherwise be obscured by the presence of reduced and denatured heavy and light chain immunoglobulin in the blot (as detected by the conventional immunoblotting HRP anti-mouse IgG reagent). Mouse IgG TrueBlot® ULTRA is the unique horseradish peroxidase conjugated anti-mouse IgG immunoblotting second step reagent which enables detection of Akt immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your usual HRP anti-mouse IgG immunoblotting second step reagent. It is easy to generate publication-quality Akt IP Western Blot data with Mouse IgG TrueBlot® ULTRA.

 


Mouse IgG TrueBlot ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in Akt IP Western Blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.

 

IP / Western Blotting comparison of TrueBlot and conventional reagents