Rockland offers a variety of antibodies to be used as markers of protein localization, purification of recombinant proteins, tag detection and signal amplification. These are called epitope tags and are generally composed of 10-15 amino acids. Epitope tags are engineered to minimize tertiary structure disruptions through on the N- or C- terminus, ensuring proper protein function. Assays focused on GFP Western Blot antibodies help researchers identify these areas of localization, making GFP microscopy extremely useful in cancer research.
HA Epitope Tag (RABBIT) Antibody
6X HIS Epitope Tag (MOUSE) Monoclonal Antibody DyLight™ 649 Conjugated
Antibodies raised against epitope tags including FLAG™, His6, cMYC, HA and V5 as well as carrier proteins GST and MBP facilitate methods of protein localization, protein topological studies and purification of recombinant proteins. Epitope tags or carrier proteins may be added at either N- or C-terminal ends of the protein-of-interest, which upon cleavage at integrated enzymatic cleavage sites yield pure recombinant protein by removal of the tag or carrier. Read more about Epitope Tags.
Green fluorescent protein (GFP) and Red fluorescent protein (RFP) are ~29 kDa fluorescent proteins used as versatile markers for visualization of protein localization, monitoring physiological processes and for detecting transgenic or transient protein expression. GFP and RFP can be used for the generation of fusion proteins without significantly interfering with native protein assembly and function, making them powerful tools for in vivo analyses. Read more about our GFP antibodies
Biotin or vitamin B7 is generally used to label proteins, including antibodies used in immunoassays. However, given the rather small size of this molecule, the overall functionality of antibodies used in the biotinylation process is not affected and labeled antibodies are capable of binding streptavidin or avidin conjugated chromogenic or fluorescent reporters in assays as described above. Biotinylated proteins of interest can be purified using monomeric avidin coated beads, thus minimizing protein denaturation upon breakage to the non-covalent bond in presence of excess biotin.
Fluorescent tags including Fluorescein, Phycoerythrin, Cyanine or Rhodamine are usually conjugated to specific antibodies for integration into platform-specific assays. These fluorescent molecules are used in the cell labeling process; however, antibodies raised against them are employed for tag detection and signal amplification.
Signal Enhancing antibodies include anti-KLH antibodies, anti-HRP (anti-Peroxidase) antibodies and anti-Luciferase antibodies. Rockland manufactures many signal enhancing antibodies in conjugated forms such as Fluorescein (FITC), Texas Red, Biotin, Alkaline Phosphatase, Rhodamine and IRDye.
In nature, Avidin is produced in the oviducts of birds, reptiles and amphibians in either dimeric or tetrameric form. In tetrameric form, Avidin is comprised of four identical subunits (homotetramer) that is capable of non-covalently binding biotin or Vitamin B7 with a great affinity and specificity. Its application in the scientific realm encompasses the conjugation of different reporters to Avidin, thus facilitating chromogenic or chemifluorescent signal enhancement when applied to biotinylated antibodies in an assay.Similarly Streptavidin, a related protein thought to be involved in bacterial defense mechanisms, displays an equal affinity for the sequestration of biotin. As with Avidin, Streptavidin is predominantly used for signal amplification purposes in an assay incorporating biotinylated antibodies.
Antibodies raised against Protein A or G can be employed to determine purified recombinant protein concentrations. Protein A (Staphylococcus aureus-derived) and Protein G (Streptococci-derived) can be used for the signal amplification of conjugates. Protein G displays a high affinity to the Fc portion of various host isotypes including human, mouse, rat, guinea pig, rabbit, goat, cow, sheep, as well as horse and unlike Protein A can bind both the Fc and Fab regions – further simplifying the purification of F(ab’) fragments generated upon papain digestion.
Rockland is focused on developing products that provide outstanding results that lead to innovative discoveries. Our quality assurance process is accurate and consistent, ensuring that the data produced by our antibodies can withstand the test of time. Trust your results to Rockland. Compromise elsewhere.
Common applications for Rockland GFP and Epitope antibody products include:
For more information or a custom quote, contact us today.
Rockland Immunochemicals Inc.Limerick, PA 19468E-mail: firstname.lastname@example.orgPhone: 800.656.7625