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Rockland produces antibodies to Green Fluorescent Protein (GFP) which are both monoclonal and polyclonal and are raised against full length GFP protein. Anti-GFP antibodies are also conjugated to Peroxidase (HRP), BIOTIN, and fluorescent dyes such as Atto Dye, FITC, IRDye and DyLight Dye. GFP is a powerful tool for monitoring gene expression and protein localization in living cells.
Rockland incorporates scientific expertise with customized protocols and proven development methods to provide seamless and reliable anti GFP antibody development for any application. Anti-GFP antibodies are suitable for the detection of native GFP, GFP variants such as S65T-GFP, RS-GFP, YFP and EGFP and most other GFP fusion proteins by ELISA, Flow Cytometry, Immunofluorescent Microscopy, Immunohistochemistry, Immunoprecipitation and western blot antibody analysis.
Applications Key/Anti-GFP: E - Elisa, FLISA - Fluorescence Based Plate Assays,IF - Immunofluorescence, IHC - Immunohistochemistry, WB - Western Blotting
For more information or to learn more about how Rockland’s Anti-GFP solutions can meet your needs, contact us today.
The Mouse TrueBlot® Western Blot Kit contains the critical supporting reagents, buffers, and substrates for AKT immunoprecipitation and GFP Western blotting of samples using TrueBlot second step immunoblotting reagents in conjunction with your own primary IP antibody and primary (Mouse IgG) Western blotting antibody. TrueBlot technology enables unhindered detection of protein bands of interest which would otherwise be obscured by the presence of reduced and denatured heavy and light chain immunoglobulin in the blot (as detected by the conventional immunoblotting HRP anti-mouse IgG reagent). Mouse IgG TrueBlot® ULTRA is the unique horseradish peroxidase conjugated anti-mouse IgG immunoblotting second step reagent which enables detection of GFP immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your usual HRP anti-mouse IgG immunoblotting second step reagent. It is easy to generate publication-quality GFP IP Western Blot data with Mouse IgG TrueBlot® ULTRA.
Mouse IgG TrueBlot ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in GFP IP western blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.