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        Horseradish Peroxidase (HRP) Secondary Antibodies


Horseradish Peroxidase (HRP) Secondary Antibodies Overview

 

 

Rockland Immunochemicals Inc., offers a wide range of stabilized HRP secondary antibody conjugates that include anti-bovine, anti-cat, anti-chicken, anti-dog, anti-donkey, anti-goat, anti-guinea pig, anti-hamster, anti-horse, anti-human, anti-monkey, anti-mouse, anti-rabbit, anti-rat, anti-sheep, anti-swine secondary antibodies that are conjugated to HRP. Furthermore, HRP secondary antibody conjugates have been engineered to recognize different types of Ig such as IgM, IgA and IgG as well as their isotypes and protein domains. Examples of anti-mouse secondary antibody HRP conjugates are anti-mouse IgG (H&L) that recognizes both heavy and light chains; anti-mouse IgG F(c) recognizing only F(c) domain, anti-mouse IgG F(ab)2 that is specific to F(ab)2 domain; anti-mouse IgG –  γ chain specific;  anti-mouse IgM – µ chain specific; anti-mouse IgA - α chain specific, anti-mouse kappa chain specific, anti-mouse lambda chain specific; anti-mouse IgG1, anti-mouse IgG2a, anti-mouse IgG2b and anti-mouse IgG3.
 
All HRP secondary conjugates are supplied as lyophilized powders for a long-term storage. Reagents can be reconstituted by adding distilled water to the final concentration 1mg/mL stocks. The working HRP conjugate solution for ELISA is normally in the range of 1:10,000 diluted in the HRP Diluent (MB-060-1000) or in ratio specified in the data sheet for each product.
 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

HRP Secondary Antibodies

 

 


 

 

 

Suggested Products for Common Assays

 

For ELISA (E), Western Blotting, Immunohistochemistry,  with mouse primary antibodies, use HRP Conjugated Anti-Mouse IgG Antibody raised in goat  (610-103-121) or in donkey (610-703-124). These antibodies have been pre-adsorbed to eliminate any potential cross-reactivities to serum proteins from other species e.g. bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep.

 

For all immunoassays with rabbit primary antibodies, use HRP conjugated Goat Ant-Rabbit IgG (611-103-122) or Donkey Anti-Rabbit IgG (611-703-127). These secondary antibodies have been pre-adsorbed to eliminate any potential cross-reactivity to serum proteins from species other than mouse.

 

When chieckn primary antibodies are used in immunoassays, use HRP conjugated anti-chicken IgG cross-adsorbed antibodies raised in goat (603-103-126). Potential cross-reactivity to undesired serum proteins has been eliminated.

 

There are a number of substrates applicable for immunoassays with HRP conjugates:

 

  • For ELISA, use colorimetric substrate (TMBE-1000) or chemiluminescent substrate (PICOMAX-110).
  • For Western Blotting use colorimetric substrate (TMBM-1000) or chemiluminescent substrate (PICOMAX-110).
  • For IHC, use 3,3' -Diaminobenzidine or DAB (DAB-10). Alternatively, there are several IHC kit developed specifically for human (KHA001), for mouse (KHA002), for rabbit (KHA003) and for goat (KHA004) primary antibodies. 

 


 

The Rockland Advantage: HRP Secondary Antibodies

 

All Rockland Immunochemicals HRP secondary antibodies are affinity-purified to assure both high affinity and specificity. To eliminate potential cross-reactivity to undesired serum proteins, secondary antibodies have been pre-absorbed. Rigorous quality control testing ensures that the finished product meets or exceeds out high standards for optimum performance in your assays. Following are our product highlights:

 

 


 

Best-Selling Rockland HRP Secondary Antibodies and Associated Reagents

 

 

Name Host Catalog # Applications Size

 

Mouse IgG (H&L) Antibody

Peroxidase Conjugated Pre-Adsorbed

 

Goat 610-103-121 ELISA, WB, IHC 1 mg

 

Mouse IgG (H&L) Antibody

Peroxidase Conjugated Pre-Adsorbed

 

Donkey 610-703-124 ELISA, WB, IHC    1 mg

 

Rabbit IgG (H&L) Antibody

Peroxidase Conjugated Pre-Adsorbed

 

Goat 611-103-122 ELISA, WB, IHC 1 mg

 

Rabbit IgG (H&L) Antibody

Peroxidase Conjugated Pre-Adsorbed

 

Donkey 611-703-127 ELISA, WB, IHC 1 mg

 

Chicken IgG (H&L) Antibody

Peroxidase Conjugated Pre-Adsorbed

 

Goat 603-103-126 ELISA, WB, IHC 1 mg

 

Chicken IgG (H&L) Antibody

Peroxidase Conjugated Pre-Adsorbed

 

Goat 600-402-379  FLISA, WB, IHC 100 μg

 

TMB ELISA Peroxidase Substrate

 

  TMBE-1000 ELISA 1 L

 

Chemiluminescent Picomax™ Sensitive HRP Substrate for Microwell and Membrane

 

  PICOMAX-110 ELISA, WB 110 mL

 

TMB Membrane Peroxidase Substrate

Ready-To-Use

 

  TMBM-1000 WB 1 L

 

DAB Membrane Peroxidase Substrate (50X)

 

  DAB-10 IHC 10 mL

 

IHC Kit for Rabbit Primary Antibody

MaxTag™ Histo 

 

  KHA003 IHC 1 Kit

  

 

 

 

Reference Rockland Bucket

 

   

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