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        Antibodies Conjugated to Infrared Dyes


Infrared Dye Overview

 

Infrared Dyes have absorption and emission wavelengths in the infrared and near-infrared (NIR) spectrum, between 680 and 800 nm. These can be used for a variety of applications, including: immunoblotting, multiplex imaging, ELISA, microscopy and small animal in vivo imaging.

 

Infrared fluorescent dyes combined with NIR fluorescent imaging delivers enhanced sensitivity due to low background autofluorescence in the near-infrared region. Therefore, they have a higher signal-to-noise ratios when used in immunoassays. Rockland produces conjugates for NIR imaging with LI-COR’s IRDyes®, DyLights® and Cy Dyes®.

 

 


 

IRDye® Conjugates

 

Rockland offers antibodies conjugated to three of LI-COR's IRDye®: IRDye® 700DX, IRDye® 800CW and IRDye® 800.

 

 

Anti-Mouse IgG (H&L) Donkey Antibody

Anti-Rabbit IgG (H&L) Goat Antibody

Anti-Mouse IgG (H&L) Donkey Antibody

MOUSE IgG Secondary Antibody

IRDye700® Pre-adsorbed

RABBIT IgG (H&L) Antibody

IRDye800® Conjugated Pre-adsorbed

Mouse IgG (H&L) Antibody
DyLight™ 680 Conjugated

 
  
 
 
View IRDye 700DX Secondary Antibodies         View IRDye800 Secondary Antibodies       View IRDye 800CW Secondary Antibodies  
 
  
 

 

   

Abs (nm)

Em (nm)

MW

IRDye ® 700DX

Near Infra-Red

689

700

1,954

IRDye ® 800CW 

Infra-Red

774

800

1,166

IRDye ® 800

Infra-Red

778

806

1,166

 

IRDye® near-infrared dyes are optimized for excitation and detection in the near-infrared spectrum, 680 nm - 800 nm.

 

 

 

While designed for use in LI-COR's Odyssey and Pearl Imaging Systems, IRDye® conjugates can be used by a variety of instruments that offer near-infrared excitation and emission and provide many advantages over traditional fluorescent dyes.

 

IRDye infrared conjugated secondary antibodies are often cross-adsorbed for multiplex detection and provide high specificity for assays such as fluorescence microscopy, flow cytometry, western blotting, ELISA, multiplex assays and other array platforms. These antibody conjugates are optimized for high sensitivity and excellent signal-to-noise performance on LI-COR Odyssey™ and Pearl™ imaging systems and other detection platforms.

 

 


 

Epitope Tag Antibodies - IRDye® Conjugated

 

Rockland offers antibodies to be used as markers of protein localization, purification or recombinant proteins, tag detection, and signal amplification. These small epitope tags are generally composed of 10-15 amino acids. Epitope tags are engineered to minimize tertiary structure disruptions through on the N- or C- terminus, ensuring proper protein function. Rockland offers a variety of epitope tag antibodies conjugated to all 3 IRDye® conjugates.

 

irday-epitope-tag-button

 


 

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Reference Rockland Bucket

 

  

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Blocking Buffer for Fluorescent

Western Blotting - MB-070

 

Blocking Buffer MB 070

 

Western Blot Blocking Solution is specifically designed for western blotting using fluorochrome conjugated antibodies. 

Blocking Buffer MB-070View Product.