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LEICA Collaboration


Leica and Rockland Immunochemicals Investigate Sub-cellular Akt Localization Using STED Microscopy:

 

Fluorescence microscopy has long been established as an extremely important diagnostic tool in medical and biological research. Collaborating with Leica Microsystems, a world leader in innovative microscopy for imaging and analysis of micro- and nanostructures, Rockland Immunochemicals has developed novel fluorescent-labeled conjugates for use in Stimulated Emission Depletion, or STED microscopy.

 

Featured Products:

 

Dylight Dye of Rabbit IgG (H&L)

 

Rabbit IgG (H&L) Antibody DyLight™ 488 Conjugated Pre-Adsorbed

Immunofluorescence Microscopy of Rabbit Anti-AKT Antibody

 

AKT (RABBIT) Antibody

Rabbit IgG (H&L) Antibody ATTO 488 Conjugated Pre-Adsorbed

 

RABBIT IgG (H&L) (GOAT) Antibody ATTO 425 Conjugated

 

 

Rockland manufactures hundreds of fluorochrome conjugates including Atto-tec and DyLight™ dyes and FITC, which complement the cutting-edge STED technology, in addition to many of the classic fluorochromes such as Texas Red and Phycoerythrin. All of the conjugates are ideal for various immunofluorescence based assays including fluorescent western blotting and FLISA, as well as immunofluorescence microscopy.


A method of confocal microscopy, STED allows for assessments of protein interactions not previously visible by conventional microscopic analysis. This super-resolution, occurs below the diffraction limit which restricts most confocal based technologies and allows for live cell imaging and subcellular architecture, as well as colocalization studies.

 

Leica TCS SP8 STED

 

 

Leica TCS SP8 STED Comparison Images

Courtesy of Leica Microsystems

 

Sample courtesy of Reinhard Windoffer, RWTH Aachen University, Institute of Molecular and Cellular Anatomy, Aachen, Germany

 

 

STED, combined with next generation fluorochrome conjugates (Atto-Tec Dyes, DyLight™ Dyes) from Rockland, offer superior absorption (high extinction coefficient), high fluorescence quantum yield, and superior photostability.  Rockland DyLight™ fluorochrome conjugated secondary antibodies are used to probe the sample and Super Resolution Confocal Microscope imaging systems developed by Leica Microsystems, provide clear, accurate images.

 

 

 

 

Leica NF-kB Poster

Download the Rockland
and Leica NF-kB Poster



Leica Akt Detection Poster

Download the Rockland
and Leica Akt Detection Poster





Leica Science Lab

STED Sample Preparation Tutorial

 

 

Leica Cell Imaging Videos:


 Leica Video