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        National Cancer Institute (NCI) Collaboration


 

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Rockland Immunochemicals, partnering with the National Cancer Institute’s Center for Cancer Research, has released a series of antibodies that are involved in signal transduction and cancer research. The goal of this collaboration is to combine basic and applied research principles, working with life science researchers to develop better bioreagents that will facilitate biomedical research. These reagents assist in the understanding of cell function as it relates to cancer management.






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PRODUCT NAME

CATALOG#

APPLICATIONS 

SIZE 


Collaboration Antibodies

Anti-ABCB1 600-401-967 E, WB, IC, Other 100 µg

Anti-ABCB5 600-401-A77 E, WB, IHC, Other 100 µg

Anti-ABCB6 600-401-945 E, WB, Other 100 µg

Anti-activator of Hsp90 ATPase (AHA1) 600-401-974 E, WB, IC 100 µg

Anti-AGAP2) 200-401-946 E, WB, Other 100 µg

Anti-Ankrd26 600-401-917 E, WB 100 µg

Anti-ASAP1 pan 600-401-911 E, WB 100 µg

Anti-ASAP1 pY782 600-401-910 E, WB, IC 100 µg

Anti-BORIS 600-401-907 E, WB, Other 100 µg

Anti-C/EBP delta 600-401-A61 E, WB, IP, Other 100 µg

Anti-c-Met pY1349 pY1306 600-401-989 WB 100 µg

Anti-c-Myb 600-401-B06 E, WB, Other 100 µg

Anti-Cas5 600-401-948 E, WB, Other 100 µg

Anti-CBLC 600-401-889 E, WB, IHC, Other 100 µg

Anti-CD97 200-401-908 E, WB, IFM, Other 500 µg

Anti-CDK9 pT29 600-401-996 E, WB, IP 100 µg

Anti-Cenexin1 200-401-C43 E, WB, Other 100 µg

Anti-Cenexin1 600-401-A46 E, WB, ICH, Other 100 µg

Anti-Cenp-Q 200-401-B48 E, WB, Other 100 µg

Anti-cKrox 100-401-A21 E, WB, IC 100 µl

Anti-cripto-1 (TNGF1) 600-401-997 E, WB, IHC, Other 100 µg

Anti-Cybr 600-401-A23 E, WB 100 µg

Anti-Cybr 100-401-A23 E, WB, IP 100 µg

Anti-Cyclin E2 600-401-971 E, WB, IP 100 µg

Anti-Daxx 600-401-966 E, WB, IFM 100 µg

Anti-ECT2 pY790 600-401-930 E, WB, IHC, Other 100 µg

Anti-ECT2 pY790 600-401-935 E, WB, Other 100 µg

Anti-eIF3S6/Int6 600-401-A62 E, WB, Other 100 µg

Anti-FAP 600-401-A73 E, WB 100 µg

Anti-FKBP8 200-401-A58 E, WB, IC 100 µg

Anti-FIV Matrix p15 600-401-992 E, WB 100 µg

Anti Glypican-1 200-401-B44 E, WB, Other 100 µg

Anti-Gpx4 600-401-972 E, WB, IC 100 µg

Anti-H2AvD pS137 600-401-914 E, WB, Other 100 µg

Anti-Hice1 109-401-C39 E, WB, Other 100 µL

Anti-Hice1pS70 600-401-C53 E, WB 100 µg

Anti-HIF-1 alpha OH Pro564 100-401-A25 E, WB, IC 100 µl

Anti-HMGN pS20/pS24 600-401-898 E, WB, IHC 100 µg

Anti-Hsp90 acetyl specific k294 600-401-981 E, WB, IHC 100 µg

Anti-Hsp90 pan 600-401-929 E, WB, IHC, Other 100 µg

Anti-IL-7 pan 600-401-A48 E, WB, IHC, Other 100 µg

Anti-IL-7 receptor chain pY449 600-401-A49 E, WB, IHC, Other 100 µg

Anti-KSHV ORF57 600-401-A94 E, WB, IC 100 µg

Anti-L1 ORF2 200-901-B20 E, WB, Other 100 µg

Anti-LacI 600-401-B04 E, WB, Other 100 µg

Anti-MAGP-2 600-401-937 E, WB, Other 100 µg

Anti-MARK2 pT595 600-401-895 E, WB, IFM, Other 100 µg

Anti-MCM2 600-401-922   E, WB 100 µg

Anti-Mect1 600-401-936 E, WB, Other 100 µg

Anti-MLF1 c-term 600-401-A91 E, WB, IC 100 µg

Anti-MLF1 n-term 600-401-A92 E, WB, IC 100 µg

Anti-MLF1 pan (internal) 600-401-923 E, WB, IC 100 µg

Anti-MLF1 pT78 600-401-930 E, WB, IHC, Other 100 µg

Anti-Mre11 600-401-896 E, WB 100 µg

Anti-Myosin 1G 600-401-959 E, WB, IP 100 µg

Anti-NCOA3 600-401-A40 E, WB, IP 100 µg

Anti-Nedd1 109-401-C38 E, WB, Other 100 µL

Anti-NFKBp65 (RelA) pS536 600-401-265 E, WB, IHC, Other 100 µg

Anti-NOXO1 600-401-899 E, WB, IHC, Other 100 µg

Anti-PBK1 600-401-A24 E, WB, IP 100 µg

Anti-PDCD4 pan 600-401-965 E, WB, IHC, Other 100 µg

Anti-PDCD4 pS457 600-401-964 E, WB, IHC, Other 100 µg

Anti-PKC beta 600-401-A76 E, WB, Other 100 µg

Anti-Pogz 600-401-A89 E, WB 100 µg

Anti-Rab11-FIP3 600-401-994 E, WB, IC 100 µg

Anti-RFFL 200-401-932 E, WB, IC 500 µg

Anti-Rho-associated Protein Kinase-2 (ROCK2) pY256 600-401-998 E, WB, IHC, Other 100 µg

Anti-RNF25 600-401-969 E, WB, IP 100 µg

Anti-RNF34 200-401-933 E, WB, IFM 500 µg

Anti-RREB1 200-401-909 E, WB, Other 500 µg

Anti-s. cerevisiae Mer2 pan 600-401-925 E, WB, IP 100 µg

Anti-s. cerevisiae Mer2 pS30 600-401-924 E, WB, IP 100 µg

 Anti-Sel W 600-401-A29 E, WB, IP, Other 100 µg

Anti-Sipa 1 600-401-A36 E, WB, IC 100 µg

Anti-Smad2 600-401-A59 E, WB 100 µg

Anti-Smad3 (internal) 600-401-973 E, WB, Other 100 µg

Anti-Smad3 (internal 179) 600-401-E70 E, WB, Other 100 µg

Anti-Smad3 pan (c-term) 600-401-920 E, WB, IHC, Other 100 µg

Anti-Smad3 pS423 pS425 600-401-919 E, WB, IHC, Other 100 µg

Anti-Smad3 pT179 600-401-C48 E, WB, Other 100 µg

Anti-Smad4 600-401-A41 E, WB 100 µg

Anti-SPANX-C 200-401-915 E, WB, IC 500 µg

Anti-SPANX pan reactive 600-401-916 E, WB 100 µg

Anti-SPS1 100-401-A22 E, WB, IP 100 µL

Anti-SPS2 200-401-999 E, WB, IP 100 µg

Anti-Stat1 600-401-A42 E, WB 100 µg

Anti-Stat1 pY701 200-401-A43 E, WB 100 µg

Anti-Stat2 pY690 600-401-A93 E, WB, Other 100 µg

Anti-Stat5 pan 600-401-A44 E, WB, Other 100 µg

Anti-Stat5 pY694 600-401-A45 E, WB, IHC, Other 100 µg

Anti-TAF1 600-401-995 E, WB 100 µg

Anti-Tamalin 600-401-988 E, WB, IP, Other 100 µg

Anti-THRA 600-401-A38 E, WB, IP 100 µg

Anti-THRB1 600-401-A96 E, WB, IP, Other 100 µg

Anti-TMBIM1 200-401- A57 E, WB, Other 100 µg

Anti-TrkCT1 600-401-993 E, WB, IP 100 µg

Anti-Ube2j1 600-401-991 E, WB, IP 100 µg

Anti-Ube2j2 600-401-B43 E, WB, Other 100 µg

Anti-UPLC1/ASAP3 200-401-990 E, WB 100 µg

$89 Trial Size Antibodies

Anti-ABCB1 600-401-967S E, WB, IHC, Other 25 µL

Anti-ABCB5 600-401-A77S E, WB, IHC, Other 25 µL

Anti-C/EBP delta 600-401-A61S E, WB, IP, Other 25 µL

Anti-c-Met pY1349 pY1356 600-401-989S E, WB, Other 25 µL

Anti-c-Myb 600-401-B06S E, WB, Other 25 µL

Anti-cripto-1 (TNGF1) 600-401-997S E, WB, IHC, Other 25 µL

Anti-Cybr 600-401-A23S E, WB, IHC, Other 25 µL

Anti-eIF3S6/Int6 600-401-A62S E, WB, Other 25 µL

Anti-FAP 600-401-A73S E, WB, Other 25 µL

Anti-FKBP8 200-401-A58S - 25 µl

Anti-Hice1 109-401-C39S E, WB, Other 25 µL

Anti-Hice1pS70 600-401-C53S E, WB, Other 25 µL

Anti-KSHV ORF57 600-401-A94S E, WB, IHC, Other 25 µL

Anti-LacI 600-401-B04S E, WB, Other 25 µL

Anti-MLF1 c-term 600-401-A91S E, WB, IHC, Other 25 µL

Anti-MLF1 n-term 600-401-A92S E, WB, IHC Other 25 µL

Anti-NCOA3 600-401-A40S E, WB, IP, Other 25 µL

Anti-Pogz 600-401-A89S E, WB, Other 25 µL

Anti-Smad2 600-401-A59S E, WB, Other 25 µL

Anti-Smad3 pS423 pS425 600-401-919S E, WB, IHC, Other 25 µL

Anti-Stat2 pY690 600-401-A93S E, WB, Other 25 µL

Anti-THRA 600-401-A38S E, WB, IP, Other 25 µL

Anti-THRB1 600-401-A96S E, WB, IP, Other 25 µL

Anti-Ube2j1 600-401-991S E, WB, IP, Other 25 µL




Applications Key: E - Elisa, IF - Immunofluorescence, IHC - Immunohistochemistry, WB - Western Blotting, IP - ImmunoPrecipitation, IF - Microscopy, IC - Immunochemistry
 



  



 

TrueBlot® Mouse
Western Blot kit

TrueBlot AKT IP Western Blotting Kit  

 

The Mouse TrueBlot® Western Blot Kit contains the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot second step immunoblotting reagents in conjunction with your own primary IP antibody and primary (Mouse IgG) Western blotting antibody. TrueBlot technology enables unhindered detection of protein bands of interest which would otherwise be obscured by the presence of reduced and denatured heavy and light chain immunoglobulin in the blot (as detected by the conventional immunoblotting HRP anti-mouse IgG reagent). Mouse IgG TrueBlot® ULTRA is the unique horseradish peroxidase conjugated anti-mouse IgG immunoblotting second step reagent which enables detection of immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your usual HRP anti-mouse IgG immunoblotting second step reagent. It is easy to generate publication-quality IP Western Blot data with Mouse IgG TrueBlot® ULTRA.

 

 

Mouse IgG TrueBlot ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP Western Blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.

  

AKT IP / Western Blotting comparison of TrueBlot and conventional reagents