25% Off Phospho Specific Antibodies
AKT pS473 (RABBIT) Antibody
AKT pT308 (RABBIT) Antibody
AKT pT308 (MOUSE) Monoclonal Antibody DyLight™ 549 Conjugated
Isoforms of AKT are found to be distinct with regard to tissue expression, pathway activation and inhibitor sensitivity. Rockland has created a panel of monoclonal antibodies to AkT isoforms. These anti-AKT antibodies are available to academic researchers as well as BioPharma companies who wish to manufacture highly sensitive pharmacodynamic assays for measuring both total Akt, its isoforms and levels of phosphorylated Akt in response to mTOR / Akt inhibitors and modulators.
AKT Signaling Antibody Applications
AKT Western Blot analysis
AKT Multiplexing Assay (, 187 kb)
The Mouse TrueBlot® Western Blot Kit contains the critical supporting reagents, buffers, and substrates for Akt immunoprecipitation and Western blotting of samples using TrueBlot second step immunoblotting reagents in conjunction with your own primary IP antibody and primary (Mouse IgG) Western blotting antibody. TrueBlot technology enables unhindered detection of protein bands of interest which would otherwise be obscured by the presence of reduced and denatured heavy and light chain immunoglobulin in the blot (as detected by the conventional immunoblotting HRP anti-mouse IgG reagent). Mouse IgG TrueBlot® ULTRA is the unique horseradish peroxidase conjugated anti-mouse IgG immunoblotting second step reagent which enables detection of Akt immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your usual HRP anti-mouse IgG immunoblotting second step reagent. It is easy to generate publication-quality Akt IP Western Blot data with Mouse IgG TrueBlot® ULTRA.
Rockland Immunochemicals Inc.Gilbertsville, PA 19525E-mail: firstname.lastname@example.orgPhone: 800.656.7625