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        PI3K / AKT Signaling


   

AKT Signaling Pathway Overview

 

AKT is one of the most actively studied kinase pathways in the basic research and drug development arenas, as it plays an integral role in mediating signals for cell growth, survival, cell-cycle progression, differentiation, transcription, translation, and glucose metabolism. Recent advances in AKT signaling have focused on understanding cellular processes and identifying cellular substrates that are physiologically relevant in vivo. These efforts have uncovered important roles for AKT pathway regulation in cancer research, neuroscience, and disease prevention. 

 

 



           

 

AKT Antibodies & Assays for Cell Signaling

 

Activation of AKT results in phosphorylation of a wide range of proteins downstream of the AKT pathway, making AKT Western Blotting a popular technique for monitoring this reaction. The phosphorylated substrates, present in various subcellular locations play an important role in directing different phenotypic outcomes. Faulty or aberrant activation of AKT underlies the pathophysiological properties of a variety of complex diseases, including type-2 diabetes, HIV and cancer. When constitutively active, AKT is utilized frequently by cancer cells to circumvent therapeutic intervention, promoting cellular survival and resistance to chemo and radiation therapy. Rockland's Anti-AKT antibodies and PI3Kinase Pathway reagents help monitor levels of AKT and its phosphorylation at position S473, T308, as well as AKT antibody isoforms AKT2 and AKT3.  
 


               

Featured Rockland AKT Antibodies:       


 

Immunoassays for AKT Signaling

There are a variety of immunoassays that can assist in monitoring AKT antibody levels, many of which are offered by Rockland.  Rockland also has the ability to develop custom assays based on specific applications. The development of a customized immunoassay is initiated by user-defined specifications pertaining to overall research-related design goals and is a process characteristically divided into 4 phases, as illustrated in this paper on assay development monograph.

Additionally, Rockland has partnered with other leading researchers to develop highly specialized Western Blot assays that simultaneously detect several human serum proteins and resting AKT1 and activated phosphorylated AKT1Read more about Akt Multiplexing Assay.

With over 20 years of experience in the development and validation of multiple assay formats, Rockland demonstrates consistent success in delivering customer solutions and possesses the expertise in optimizing every aspect of an immunoassay.

 


 

Pharmacodynamic Assay for Akt / mTOR Inhibitors

 

 

Isoforms of AKT are found to be distinct with regard to tissue expression, pathway activation and inhibitor sensitivity. Rockland has created a panel of monoclonal antibodies to AkT isoforms. These anti-AKT antibodies are available to academic researchers as well as BioPharma companies who wish to manufacture highly sensitive pharmacodynamic assays for measuring both total Akt, its isoforms and levels of phosphorylated Akt in response to mTOR / Akt inhibitors and modulators.

 

 


 

Continued AKT Antibody Research

 

Rockland continues to innovate and drive thought leadership in antibody science, as well as the technology important to the future of the industry. Stimulated Emission Depletion microscopy, or STED microscopy, is a technique that uses the non-linear de-excitation of fluorescent dyes to overcome the resolution limit imposed by diffraction with standard confocal laser scanning microscopes and conventional far-field optical microscopes. Compared to traditional confocal microscopy, STED offers exceptional improvements in resolution allowing visualization of cellular events at unprecedented levels.

Rockland and Leica Microsystems have jointly analyzed the sub-cellular localization of AKT using both standard confocal microscopy and high resolution STED nanoscopy. The results and findings are presented in this Akt poster, with the obvious STED improvement demonstrated throughout.
 
 

 

AKT Signaling Antibody Applications

 

 

AKT Western Blot analysis

 

  

AKT Immunoprecipitation

 

 

AKT ELISA  

 

 

AKT Immunohistochemistry  

 

 

AKT Immunofluorescence  

 

Related Products:

 

Beta-Amyloid Antibodies

 

 

Resources:

 

AKT Pathway Poster ( AKT Pathway Poster, 5.2 mb)  
 

 
AKT Multiplexing Assay (AKT Multiplexing Assay, 187 kb)   

 
 

Clickable Akt signaling pathwayAkt button

 

 

 

                                   

Akt Antibody Signaling Pathway Poster

Akt Signaling Pathway
 

 

  



  

 

TrueBlot® Mouse
Western Blot Kit

 

TrueBlot IP Western Blotting Kit

The Mouse TrueBlot® Western Blot Kit contains the critical supporting reagents, buffers, and substrates for Akt immunoprecipitation and Western blotting of samples using TrueBlot second step immunoblotting reagents in conjunction with your own primary IP antibody and primary (Mouse IgG) Western blotting antibody. TrueBlot technology enables unhindered detection of protein bands of interest which would otherwise be obscured by the presence of reduced and denatured heavy and light chain immunoglobulin in the blot (as detected by the conventional immunoblotting HRP anti-mouse IgG reagent). Mouse IgG TrueBlot® ULTRA is the unique horseradish peroxidase conjugated anti-mouse IgG immunoblotting second step reagent which enables detection of Akt immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your usual HRP anti-mouse IgG immunoblotting second step reagent. It is easy to generate publication-quality Akt IP Western Blot data with Mouse IgG TrueBlot® ULTRA. 

  

IP / Western Blotting comparison of TrueBlot and conventional reagents