Rockland offers a
variety of purification services for the purification of native and recombinant
proteins, as well as for antibodies (including fragments and modified proteins).
Native and recombinant
protein purification can be carried out on materials that you supply or are generated by
Rockland through our contract services. These proteins can also be further
modified by chemical or enzymatic treatments (crosslinking, digestions, etc)
and further purified if warranted.
Highly purified proteins
may be critical to your work, and this is particularly true for the generation
of antibodies. Antibody preparations will often contain a number of substances
from the production process that can adversely affect the quality of your research
results. The best way to ensure accurate findings is to purify your antigens
and antibodies prior to use. The following are examples of Rockland’s purification
Polyclonal and monoclonal antibodies are commonly purified by Protein A, Protein G or Protein A/G affinity chromatography under optimal conditions based on isotype specificity. To further improve the quality of your antibody preparations, Rockland can also generate custom resins for the immobilization of target-specific antigens (i.e., chemical moieties, peptides or recombinant proteins) for the affinity purification of generated antiserum and for serum depletion. Sample gels of purified antibodies are provided below. To learn more about our purification services or to obtain a quote, contact us today.
Mammalian antibodies are commonly purified using Protein A, Protein G, or Protein A/G affinity chromatography. The optimal conditions for purification depend on the antibody isotype. Other antibody binding moieties (e.g., Protein L, which binds to the antibody kappa chain) may also be used.
For example, protein A/G binds to all subclasses of human IgG, making it useful for purifying polyclonal or monoclonal IgG antibodies whose subclasses have not been determined. Additional subclasses to which it binds, include: IgA, IgE, IgM and IgD. Protein A/G also binds to all subclasses of mouse IgG but does not bind mouse IgA, IgM or serum albumin. The appropriate antibody-binding protein thus requires consideration of the antibody species of interest.
Rockland also offers the generation of custom resins for the immobilization of target-specific compounds, peptides or recombinant proteins for the affinity purification of antibodies from antiserum or other fluids (e.g., plasma, serum, other fluid or tissue extracts, and tissue culture or microbial supernatants and extracts). Such custom resins can also be used for antibody depletion from these preparations. Contact us to discuss your specific challenges and needs, and exploration of a path forward to successfully carry out your research projects.
Recombinant Fusion proteins or epitope tagged proteins can be purified by affinity chromatography using charged or immobilized resins. In addition, purificaiton services by Ion Exchange or Size Exclusion HPLC is also offered by Rockland to ensure delivery of recombinant proteins of exceptional purity.
Rockland Immunochemicals Inc.Limerick, PA 19468E-mail: email@example.comPhone: 800.656.7625