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Primary Antibodies  >  Phospho Specific Antibodies
 

AKT phospho T308 Antibody

Rabbit Polyclonal
Code

600-401-269
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western Blot of Rabbit anti-Akt phospho T308 antibody.  
Lane 1:  GST tagged AKT1 un-active recombinant protein.  
Lane 2:  GST tagged AKT1 active recombinant protein.  
Load:  50 ng per lane.
Primary antibody:  Akt phospho T308 antibody at 1:1,000 for overnight at 4°C.
Secondary antibody:  DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT.
Block:  MB-070 for 30 min at RT.
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1

AKT phospho T308 Antibody Properties

Description
Anti-AKT pT308 (RABBIT) Antibody - 600-401-269
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Application
ELISA : 1:15,000
FlowCytometry : User Optimized
Western Blot : User Optimized
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
.44 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
AKT1
Download CofA
Lot#  

AKT phospho T308 Antibody Description

Background
AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at  Ser 473 and Thr 308.  AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways.  AKT Exhibits tight control over cell proliferation and cell viability.  Overexpression or inappropriate activation of AKT is noted in many types of cancer.   AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.  AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
Immunogen
Anti-AKT phospho T308 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to aa 302-311 of human AKT protein conjugated to KLH.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store AKT phospho T308 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
AKT antibody is phospho specific for pT308 and is suitable for western blotting and ELISA.
Purity/Specifity
AKT phospho T308 Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Rabbit Serum.  This antibody is specific for phosphorylated human AKT.  Minimal reactivity occurs against non-phosphorylated AKT.  Reactivity against AKT from other species may occur but has not yet been tested.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Lawlor, M. A. and Alessi, D.R. (2001). PKB/AKT: a key mediator of cell proliferation, survival and insulin responses.  J. Cell Science 114:2903-2910. Alessi, D. R. (2001). Discovery of PDK1, one of the missing links in insulin signal transduction. Biochem. Soc. Trans. 29,1 -14. Hedjazifar,S., Jenndahl,L.E., Shimokawa,H. and Baeckstrom,D. (2005) PKB mediates c-erbB2-induced epithelial beta1 integrin conformational inactivation through Rho-independent F-actin rearrangements. Exp. Cell Res. 307 (1), 259-275. Saji,M., Vasko,V., Kada,F., Allbritton,E.H., Burman,K.D. and Ringel,M.D. (2005) Akt1 contains a functional leucine-rich nuclear export sequence.  Biochem. Biophys. Res. Commun. 332 (1), 167-173. Sutherland,B.W., Kucab,J., Wu,J., Lee,C., Cheang,M.C., Yorida,E., Turbin,D., Dedhar,S., Nelson,C., Pollak,M., Leighton Grimes,H., Miller,K., Badve,S., Huntsman,D., Blake-Gilks,C., Chen,M., Pallen,C.J. and Dunn,S.E. (2005) Akt phosphorylates the Y-box binding protein 1 at Ser102 located in the cold shock domain and affects the anchorage-independent growth of breast cancer cells.  Oncogene 24 (26), 4281-4292.
Specific Reference
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Product Type
Proteins and Peptides;
Catalog Number
000-000-401

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-269

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
human, mouse, rat, chimpanzee
Catalog Number
200-301-401

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry