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GFP and Epitopes  >  Antibodies to GFP and Antibodies to RFP
 

GFP Antibody Fluorescein Conjugated Pre-adsorbed

Goat Polyclonal
Code

600-102-215
Size

1 mg
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Immunofluorescence Microscopy of GFP-GOAT-Antibody.  
Tissue:  Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification).  
Fixation:  4%PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution.
Antigen retrieval:  frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns.
Primary antibody: Goat anti-GFP was used at 1:500 dilution in free floating imunnohistochemistry to detect GFP.
Secondary antibody:  Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1:10,000 for 45 min at RT.
Localization:  Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus.
Staining:  eGFP as green fluorescent signal and sections were counterstained with DAPI.
Western Blot of Goat anti-GFP Fluorescein Conjugated Antibody.  
Lane 1:  GFP.  
Lane 2:  None.  
Load:  50 ng per lane.
Primary antibody:  None.
Secondary antibody:  Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT.
Block:  MB-070 for 30 min at RT.
Predicted/Observed size:  28 kDa, 33 kDa for GFP. 
Other band(s): None.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: FITC, GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.

GFP Antibody Fluorescein Conjugated Pre-adsorbed Properties

Description
Anti-GFP (GOAT) Antibody Fluorescein Conjugated Min X Hu Ms and Rt Serum Proteins - 600-102-215
Host
Goat
Known Cross Reactivity
wt, rGFP, eGFP
Clonality
Polyclonal
Application
FLISA : >1:20,000
IF Microscopy : 1:500 - 1:2,500
Western Blot : >1:10,000
Other Dilution: User Optimized
Epitope Tag Type
GFP
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
FP Value
2.9 moles Fluorescein (FITC) per mole of IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Lot#  

GFP Antibody Fluorescein Conjugated Pre-adsorbed Description

Background
GFP Fluorescein Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Immunogen
Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogen Type
Recombinant Protein
Storage Condition
Store anti-GFP at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Purity/Specifity
GFP Fluorescein Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Fluorescein and purified and partially purified Green Fluorescent Protein (Aequorea victoria).  No reaction was observed against Human, Mouse and Rat Serum Proteins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
Kofidis, T., de Bruin, J. L., Yamane, T., Tanaka, M., Lebl, D. R., Swijnenburg, R. J., ... & Robbins, R. C. (2005). Stimulation of paracrine pathways with growth factors enhances embryonic stem cell engraftment and host-specific differentiation in the heart after ischemic myocardial injury. Circulation, 111(19), 2486-2493. Post, H., Kajstura, J., Lei, B., Sessa, W. C., Byrne, B., Anversa, P., ... & Recchia, F. A. (2003). Adeno-associated virus mediated gene delivery into coronary microvessels of chronically instrumented dogs. Journal of Applied Physiology, 95(4), 1688-1694. Hashimoto, N., Jin, H., Liu, T., Chensue, S. W., & Phan, S. H. (2004). Bone marrow–derived progenitor cells in pulmonary fibrosis. Journal of Clinical Investigation, 113(2), 243-252.
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Fluorescein (FITC)
Color
Green
Conjugation Reference
Modified from The and Feltkamp, Immunology 18; 865, 1970.
Molecular Weight
390
Excitation Wavelength
495
Conjugation Chemistry
Isothiocyanate