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GFP and Epitopes  >  Antibodies to GFP and Antibodies to RFP
 

GFP Antibody IRDye® 800 Conjugated

Goat Polyclonal
Code

600-132-215
Size

500 µg
Price

$309.00
Availability

Ships next business day.
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Western Blot of Anti-Green Fluorescent Protein antibody.  
Lane 1:  MW.  
Lane 2:  50ng of GFP.  
Primary antibody:  none.
Secondary antibody: Anti-Green Fluorescent Protein goat Antibody IRDye800® Conjugated (p/n 600-132-215) secondary antibody was used at 1:5,000 for 45 min at RT  in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070).
Block:  5% Blotto (p/n B501-0500) 1 Hour at 20°C.
Predicted/Observed size:  27 kDa for GFP. 
Other band(s): none.
Western Blot of anti-alpha tubulin antibody.  
Lane 1:  HeLa lysate.  
Lane 2:  NIH/3T3 lysate.  
Load:  35 µg per lane.
Primary antibody:  alpha tubulin antibody at 1:2500 for overnight at 4°C.
Secondary antibody:  IRDye800®  secondary antibody was used at 1:20,000 for 45 min at RT.
Block:  Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) overnight at 4°C.
Predicted/Observed size:  50 kDa for alpha tubulin. 
Other band(s): none.
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Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.

GFP Antibody IRDye® 800 Conjugated Properties

Description
Anti-GFP (GOAT) Antibody IRDye® 800 Conjugated Min X Hu Ms and Rt Serum Proteins - 600-132-215
Host
Goat
Known Cross Reactivity
wt, rGFP, eGFP
Clonality
Polyclonal
Application
FLISA : >1:20,000
IF Microscopy : >1:5,000
Western Blot : 1:10,000 - 1:25,000
Other Dilution: User Optimized
Epitope Tag Type
GFP
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
FP Value
4.4 moles IRDye® 800 per mole of IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
500 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Lot#  

GFP Antibody IRDye® 800 Conjugated Description

Background
GFP IRDye® 800 Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Immunogen
Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background. IRDye® 800 and IRDye® 700DX antibody and reagent conjugates are specifically designed for protein detection methods that use longer-wavelength, near-infrared (IR) fluorophores to visualize proteins in western blotting and other applications. Very low background fluorescence in the IR range provides for a much higher signal-to-noise ratio than visible fluorophores. Detection levels in the picogram range on Western blots rival the sensitivity of chemiluminescence on film. IRDye® 800 conjugates are optimized for the Odyssey® Infrared Imaging System developed by LI-COR. IRDye® 800 conjugates are also suitable for immunofluorescence microscopy using commercially available excitation/emission filters in the 780nm/820nm range. Dual simultaneous labeling in western blots or microscopy is achieved when IRDye® 800 conjugates are used in conjunction with IRDye® 700DX or DyLight™680 conjugates. IRDye® 800 and IRDye® 700DX conjugates provide an ultra-sensitive and convenient alternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging.
Purity/Specifity
GFP IRDye® 800 Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum.  No reaction was observed against Human, Mouse or Rat serum proteins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
GFP and Epitopes;
Catalog Number
000-001-379

Product Type
GFP and Epitopes;
Applications
ELISA, FLISA, WB, IF
Reacts With
wt, rGFP, eGFP
Catalog Number
200-341-215

Product Type
GFP and Epitopes;
Applications
ELISA, FLISA, WB, IF
Reacts With
wt, rGFP, eGFP
Catalog Number
200-344-215

Product Type
GFP and Epitopes;
Applications
IF
Reacts With
wt, rGFP, eGFP
Catalog Number
600-100-215

Product Label

 

Description
IRDye® 800
Color
Infra-Red
Conjugation Reference
Modified from LI-COR Biosciences, Lincoln, NE
Molecular Weight
1166
Excitation Wavelength
778
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester