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GFP and Epitopes  >  Antibodies to GFP and Antibodies to RFP
 

GFP Antibody Dylight™ 649 Conjugated Pre-Adsorbed

Goat Polyclonal
Code

600-143-215
Size

100 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Two-color Western Blot using a DyLight™ 549 and DyLight™ 649 conjugate.  
Lane 1-5:  25ng, 12ng, 6ng, 3ng, and 1ng of TNFa.  
Lane 6-11:  0.5ng, 1ng, 3ng, 6ng, 12ng, 25ng of Tubulin.  
Lane 12: Molecular Weight.
Primary antibody:  none.
Secondary antibody:  DyLight™ 549 and DyLight™ 649 mouse secondary antibody at 1:10,000.
Block:  MB-070 for 2 hrs at RT.
Predicted size:  17kDa TNFa and 55kDa Tubulin. 
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.

GFP Antibody Dylight™ 649 Conjugated Pre-Adsorbed Properties

Description
Anti-GFP (GOAT) Antibody DyLight™ 649 Conjugated Min X Hu Ms and Rt Serum Proteins - 600-143-215
Host
Goat
Known Cross Reactivity
wt, rGFP, eGFP
Clonality
Polyclonal
Application
ELISA : 1:20,000-1:40,000
FLISA : >1:10,000
IF Microscopy : >1:5,000
Western Blot : 1:10,000-1:25,000
Other Dilution: User Optimized
Epitope Tag Type
GFP
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
FP Value
3.0 moles DyLight™ 649 per mole of IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Lot#  

GFP Antibody Dylight™ 649 Conjugated Pre-Adsorbed Description

Background
GFP Dylight™ 649 Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Immunogen
The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Purity/Specifity
GFP Dylight™ 649 Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).  No reaction was observed against Human, Mouse or Rat serum proteins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
DyLight™ 649
Color
Far Red
Conjugation Reference
Modified from Thermo Fisher Scientific Inc, Rockford, IL
Molecular Weight
1008
Excitation Wavelength
646
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester