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GFP and Epitopes  >  Antibodies to GST
 

GST Antibody

Goat Polyclonal
Code

600-101-200
Size

1 mg
Price

$215.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Rockland's anti-GST polyclonal antibody in western blot shows detection of recombinant GST (indicated by band at ~ 28 kDa). The SDS-PAGE contained approximately 0.2 µg of rGST loaded on to a 4-20% gradient gel for separation.  After electrophoresis, the gel was transferred to nitrocellulose and blocked with “Blocking Buffer for Fluorescent Western Blotting” p/n MB-070 in TBS for 1h at RT. The membrane was probed with anti-GST antibody at a 1:2,000 dilution in blocking reagent, overnight at 4° C. For detection DyLight™800 conjugated Donkey-a-Goat IgG (p/n 605-745-002) was used at a 1:20,000 dilution (in blocking reagent) for 30 min at 25° C.  Fluorescent data was collected on a LICOR Odyssey instrument.
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Synonyms: GST, Glutathione-S-Transferase, Anti-GST Antibody

GST Antibody Properties

Description
Anti-GST (GOAT) Antibody - 600-101-200
Host
Goat
Clonality
Polyclonal
Application
ELISA : 1:30,000 - 1:100,000
Western Blot : 1:3,000 - 1:20,000
Immunohistochemistry: 1:1,000 - 1:10,000
ImmunoPrecipitation: User Optimized
Other Dilution: User Optimized
Epitope Tag Type
GST
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
Related Links
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Lot#  

GST Antibody Description

Background
Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.
Immunogen
Glutathione-S-Transferase [Schistosoma japonicum]
Immunogen Type
Native Protein
Storage Condition
Store GST antibody at 4° C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Application Note
Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. This antibody has also been reported to be suitable for immobilization in Label-free Interaction Analysis (Biocore).
Purity/Specifity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].  Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Sagermann M., Chapleau R.R., DeLorimier E., Lei M. (2009) Using affinity chromatography to engineer and characterize pH-dependent protein switches. Protein Sci. 18:217-228 [PubMed: 19177365] Rizzolio F, Lucchetti C, Caligiuri I, Marchesi I, Caputo M, Klein-Szanto AJ, Bagella L, Castronovo M, Giordano A. (2012) Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1. Cell Death and Differentiation 1-10. Nature
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry