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GFP and Epitopes  >  Antibodies to More Epitope Tags
 

MYC Epitope Tag Antibody

Rabbit Polyclonal
Code

600-401-381
Size

100 µg
Price

$215.00
Availability

Ships next business day.
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Anti-Myc epitope tag polyclonal antibody detects both AMINO and CARBOXY terminal linked Myc-tagged recombinant proteins by western blot.  Polyclonal rabbit host anti-Myc epitope tag antibody was diluted to 1.0 µg/ml to detect either recombinant protein.  4-20% gradient gels were used to resolve the proteins by SDS-PAGE.  The proteins were transferred to nitrocellulose using standard methods.  After blocking, the membranes were probed with the primary antibody overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code 611-132-122) for 45 min at room temperature (Green, 800 nm channel).   Pre-stained molecular weight markers are also shown (lane M, Red, 700 nm channel).  LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.  Other detection systems will yield similar results.
Anti-Myc epitope tag polyclonal antibody detects ~ 100 kDa CARBOXY terminal linked Myc-tagged recombinant protein present in ~35 µg of lysate by western blot.  Carboxy terminal linked Myc recombinant protein was the gift of Zhongsheng You, Salk Institute, LaJolla, CA.
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Synonyms:

MYC Epitope Tag Antibody Properties

Description
Anti-MYC EPITOPE TAG (RABBIT) Antibody - 600-401-381
Host
Rabbit
Clonality
Polyclonal
Application
ELISA : 1:135,000
Western Blot : 1:500 - 1:5,000
Immunochemistry: User Optimized
Other Dilution: User Optimized
Epitope Tag Type
MYC
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
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Lot#  

MYC Epitope Tag Antibody Description

Background
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein.  Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
Immunogen
This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide, E-Q-K-L-I-S-E-E-D-L, conjugated to KLH using maleimide.  The sequence corresponds to amino acids 410-419 of human c-Myc.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Myc has utility to detect the fusion protein of the Myc epitope cloned along with the target gene.   As such, anti-Myc/Myc can be used to identify fusion proteins containing the Myc epitope.  The antibody recognizes the Myc tag fused either to the AMINO- or CARBOXY- termini of targeted proteins.  This antibody is suitable for ELISA and western blotting and was tested against both the immunizing peptide and Myc-tagged recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Purity/Specifity
This affinity purified antibody is directed against human c-Myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag.  The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry