14-10_house_marker_thin
 
You may also want to consider these related products.
Anti-AKT pS473 (MOUSE) Monoclonal Antibody - 200-301-268
Anti-AKT pS473 (MOUSE) Monoclonal Antibody - 200-301-268
Anti-MOUSE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated - 610-4302
Anti-MOUSE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated - 610-4302
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302
NORMAL GOAT SERUM (NGS) - B304
NORMAL GOAT SERUM (NGS) - B304
GFP and Epitopes  >  Antibodies to More Epitope Tags
 

HA EPITOPE TAG Antibody

Rabbit Polyclonal
Code

600-401-384
Size

100 µg
Price

$215.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Rockland's Affinity Purified anti-HA epitope tag polyclonal antibody detects HA tagged recombinant proteins by IHC on formalin fixed paraffin embedded tissue.  Arrowheads point to expression of HA tagged proteins in endothelial cells of mouse aorta.  Sections of 4 µm were prepared from representative paraffin blocks. Sections were then deparaffinized and rehydrated with xylene and alcohol.  Citrate buffer antigen retrieval was performed for 30 min in a boiling jar. Anti-HA was diluted in blocking buffer at 1:2,000 and reacted at 4° C overnight followed by signal detection using horseradish peroxidase with DAB as the chromogenic substrate.  Tissue was counterstained with Mayer's hematoxylin. Personal Communication, Behzad Yeganeh,U. Manitoba, Winnipeg, Canada.
Anti-HA epitope tag polyclonal antibody detects HA-tagged recombinant proteins by western blot.  Polyclonal Rabbit anti-HA epitope tag, at a 1:2,000 dilution, was used to detect 1.0 µg of 12-Epitope Tag Protein Marker Lysate (p/n MB-301-0100) containing the HA epitope tag.   A 4-20% gradient gel was used to resolve the protein by SDS-PAGE.  The lysate was transferred to nitrocellulose using standard methods.  After blocking, the membrane was probed with Rockland's anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:20,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code 611-132-122) for 30 min at room temperature.  LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.  Other detection systems will yield similar results.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: hemagglutinin antibody

HA EPITOPE TAG Antibody Properties

Description
Anti-HA EPITOPE TAG (RABBIT) Antibody - 600-401-384
Host
Rabbit
Known Cross Reactivity
HA Tag Proteins
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:100,000
ChIP : Yes
Western Blot : 1:2,000 - 1:10,000
Immunochemistry: 1:500 - 1:2,000
ImmunoPrecipitation: Yes
Other Dilution: User Optimized
Epitope Tag Type
HA
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
Download CofA
Lot#  

HA EPITOPE TAG Antibody Description

Background
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein.   Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein when doing studies with transfected cells.
Immunogen
Anti-HA antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH using maleimide.  A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins.  As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope.  The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors.  This antibody has been tested by ELISA and western blotting against both the immunizing peptide and HA containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques.  Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.
Purity/Specifity
This affinity purified Anti-HA antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag.  To date, this antibody has reacted with all HA-tagged proteins tested.  In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Field, J., et al. (1988) Mol. Cell Biol. 8:2159-2165
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry