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APOLIPOPROTEIN E Antibody

Goat Polyclonal
NCI Collaboration
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Western Blot of Rabbit Anti Apolipoprotein E Antibody.  
Lane 1:  rat glial whole cell lysate.   
Load:  40 µg per lane.
Primary antibody:  Apolipoprotein E antibody at 1:1000 for overnight at 4°C.
Secondary antibody:  HRP rabbit secondary antibody at 1:200 for 45 min at RT.
Block:  3%BLOTTO overnight at 4°C.
Predicted/Observed size:  36 kDa, 35-36 kDa for APO-E. MW markers arrows at 25kDa and 37kDa.
Other band(s): ~55kDa.
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Number:
600-101-197
Size:
500 µg
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

APOLIPOPROTEIN E Antibody Properties

Description
Anti-Apolipoprotein E (GOAT) Antibody - 600-101-197
Host
Goat
Target Species
Human
Known Cross Reactivity
human, rat
Clonality
Polyclonal
Application
ELISA : 1:4,000 - 1:8,000
Western Blot : 1:500 - 1:2,000
Immunohistochemistry: 1:50 - 1:200
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Wet Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
APOE
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APOLIPOPROTEIN E Antibody Description

Background
Anti Apolipoprotein E Antibody recognizes the gene product of APOE. Apolipoprotein E is found in the chylomicron and IDLs that binds to a specific receptor on liver cells and peripheral cells. Apolipoprotein E is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This antibody is suitable for cardiovascular research.
Synonyms
Alzheimer disease 2 antibody, Apo E antibody, ApoE antibody, APOEA antibody, Apolipoprotein E3 antibody, ApolipoproteinE antibody, Apoprotein antibody
Immunogen
Apolipoprotein Type E was isolated from human plasma by density gradient centrifugation followed by HPLC purification.
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Application Note
Apolipoprotein E antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
Purity/Specifity
Anti-Apolipoprotein E has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.  Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards.  This antibody reacts with human apoLipoprotein E and has negligible cross-reactivity with Type A-1 A-II, B, C-I, C-II, C-III and J apoLipoproteins.  Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined.  Non-specific cross-reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Didangelos A et al. Novel Role of ADAMTS-5 Protein in Proteoglycan Turnover and Lipoprotein Retention in Atherosclerosis. J Biol Chem 287:19341-5 (2012). PubMed: 22493487. Brown MG et al. Dramatic caspase-dependent apoptosis in antibody-enhanced dengue virus infection of human mast cells. J Leukoc Biol 85:71-80 (2009). PubMed: 18809735. Atkinson KR et al. An altered pattern of circulating apolipoprotein E3 isoforms is implicated in preeclampsia. J Lipid Res : (2008). PubMed:18725658.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human
Catalog Number
600-101-109

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human
Catalog Number
600-101-110

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human
Catalog Number
600-101-111

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human
Catalog Number
600-101-112

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry