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Primary Antibodies  >  Apoptosis Antibodies
 

RNF34 Antibody

Rabbit Polyclonal
Code

200-401-933
Size

500 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's Protein A Purified anti-RNF34 antibody shows detection of human RNF34 (arrowhead) in lysate.  Lanes corresponding to empty vector 293T cell lysate (mock, left); RNF34 transfected lysate (middle) and RFFL transfected lysate (right), are shown using 20 µl of lysate per lane.  Lysates were prepared from equivalent numbers of cells.  Data presented demonstrate that this reagent is specific for RNF34.  After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000 using 5% BLOTTO, 0.1% Tween-20 in PBS as the diluent.  Incubation occurred for 1 h at room temperature. Personal Communication, Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.
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Synonyms: Caspase regulator CARP1 antibody, CARP 1 antibody, hRFI antibody, RFI antibody, RIFF antibody, Ring finger protein 34 antibody

RNF34 Antibody Properties

Description
Anti-RNF34 (RABBIT) Antibody - 200-401-933
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:4,000 - 1:20,000
IF Microscopy : User Optimized
Western Blot : 1:500 - 1:3,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Concentration
5.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
500 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
RNF34
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Lot#  

RNF34 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. RNF34, also known as RING finger protein 34, RING finger protein RIFF, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, hRFI, Caspases-8 and -10-associated RING finger protein 1, CARP-1 and Caspase regulator CARP1, is a novel modulator of NF-kB activation.  RNF34 possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-373 of human RNF34 protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This protein A purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 42 kDa in size corresponding to RNF34 protein by western blotting in the appropriate tissue or cell lysate or extract.  Isoforms 1 and 2, which are equivalent in size, should both cross-react with this antibody.
Purity/Specifity
This protein A purified antibody is directed against human RNF34 protein.  The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above.  A BLAST analysis was used to suggest cross-reactivity with RNF34 protein from orangutan (99%), bovine (91%), dog (89%), mouse (88%) and rat (85%) based on a high degree of protein:protein homology with the immunizing sequence.  Expect reactivity with isoform 1 and 2 of RNF34.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Sasaki,S., Watanabe,T., Konishi,T., Kitayama,J. and Nagawa,H. (2004) Effects of expression of hRFI on adenoma formation and tumor progression in colorectal adenoma-carcinoma sequence. J. Exp. Clin. Cancer Res. 23 (3), 507-512. Sasaki,S., Nakamura,T., Arakawa,H., Mori,M., Watanabe,T., Nagawa,H. and Croce,C.M. (2002) Isolation and characterization of a novel gene, hRFI, preferentially expressed in esophageal cancer. Oncogene 21 (32), 5024-5030.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human
Catalog Number
200-401-A34

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
Human, Mouse, Rat
Catalog Number
600-401-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat, Xenopus
Catalog Number
600-401-964

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human, mouse, rat
Catalog Number
600-401-966

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry