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Anti-DAXX (RABBIT) Antibody - 600-401-966
Anti-DAXX (RABBIT) Antibody - 600-401-966
Primary Antibodies  >  Apoptosis Antibodies
 

DAXX Antibody

Rabbit Polyclonal
Code

600-401-966
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Western blot using Rockland's affinity purified anti-DAXX antibody shows detection of DAXX in RWPE-1 cell extracts. The membrane was probed with the primary antibody diluted to 1:200, and further probed with peroxidase conjugated anti-Rabbit IgG at a
1:25,000 dilution. Personal Communication, Jie LIU,  CCR-NCI, Bethesda, MD.
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Synonyms: BING2 antibody, DAP 6 antibody, DAP6 antibody, Death associated protein 6 antibody, Death domain associated protein 6 antibody

DAXX Antibody Properties

Description
Anti-DAXX (RABBIT) Antibody - 600-401-966
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:7,000 –  1:45,000
IF Microscopy : User Optimized
Western Blot : 1:200 – 1:2000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.06 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
DAXX
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Lot#  

DAXX Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. DAXX, the Death domain-associated protein 6 (also named Fas death domain-associated protein, ETS1-associated protein 1
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 261-274 of human DAXX protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity-purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 81 kDa in size corresponding to DAXX protein by western blotting in the appropriate cell lysate or extract.
Purity/Specifity
This affinity-purified antibody is directed against human DAXX protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  A BLAST analysis was used to suggest cross reactivity with DAXX protein from human, mouse, and rat based on 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Hollenbach, AD, McPherson, CJ, Mientjes, EJ, Iyengar, R, and Grosveld, G (2002)  Daxx and histone deacetylase II associate with chromatin through an interaction with core histones and the chromatin-associated protein Dek. Journal of Cell Science 115, 3319-3330. Kim, E-J, Park, J-S, and Um, S-J (2003) Identification of Daxx interacting with p73, one of the p53 family, and its regulation of p53 activity by competitive interaction with PML. Nucleic Acids Research 31(18), 5356-5367.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human
Catalog Number
200-401-A34

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, FC
Reacts With
Human, Mouse, Rat
Catalog Number
600-401-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat, Xenopus
Catalog Number
600-401-964

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human, mouse, rat
Catalog Number
600-401-966

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry